Aerenchyma formation in roots of maize during sulphate starvation

dc.contributor.author	Bouranis, DL	en
dc.contributor.author	Chorianopoulou, SN	en
dc.contributor.author	Siyiannis, VF	en
dc.contributor.author	Protonotarios, VE	en
dc.contributor.author	Hawkesford, MJ	en
dc.date.accessioned	2014-06-06T06:45:25Z
dc.date.available	2014-06-06T06:45:25Z
dc.date.issued	2003	en
dc.identifier.issn	00320935	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00425-003-1007-6	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2433
dc.subject	Active oxygen species	en
dc.subject	Aerenchyma formation	en
dc.subject	Programmed cell death	en
dc.subject	Root aerenchyma	en
dc.subject	Sulphate starvation	en
dc.subject	Zea	en
dc.subject.other	Fluorescence	en
dc.subject.other	Growth kinetics	en
dc.subject.other	Hydrogen peroxide	en
dc.subject.other	Negative ions	en
dc.subject.other	Plant cell culture	en
dc.subject.other	Plant roots	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Acridine Orange	en
dc.subject.other	Apoptosis	en
dc.subject.other	Calcium	en
dc.subject.other	Hydrogen Peroxide	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Plant Epidermis	en
dc.subject.other	Plant Roots	en
dc.subject.other	Reactive Oxygen Species	en
dc.subject.other	Sulfates	en
dc.subject.other	Superoxides	en
dc.subject.other	Zea mays	en
dc.subject.other	Hypoderris	en
dc.subject.other	Poaceae	en
dc.subject.other	Zea	en
dc.subject.other	Zea mays	en
dc.title	Aerenchyma formation in roots of maize during sulphate starvation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00425-003-1007-6	en
heal.publicationDate	2003	en
heal.abstract	Young maize (Zea mays L., Poaceae) plants were grown in a complete, well-oxygenated nutrient solution and then deprived of their external source of sulphate. This treatment induced the formation of aerenchyma in roots. In addition to the effect of sulphate starvation on root anatomy, the presence and location of superoxide anions and hydrogen peroxide, and changes in calcium and pH were examined. By day 6 of sulphate deprivation, aerenchyma started to form in the roots of plants and the first aerenchymatous spaces were apparent in the middle of the cortex. S-starvation also induced thickening of the cell walls of the endodermis. Active oxygen species appeared in groups of intact mid-cortex cells. Formation of superoxide anion and hydrogen peroxide was found in degenerating cells of the mid-cortex. Very few nuclei in the cortex of S-starved roots fluoresced, being shrunken and near to the cell wall. By day 12 of S-deprivation, a fully developed aerenchyma was apparent and there were only a few 'chains' of cells bridging hypodermis to endodermis and stele of roots. Cell walls of endodermis of S-starved roots increased 68% in thickness. Intensive fluorescence in the cell walls of the endodermal, hypodermal and to a lesser extent of epidermal cells was observed due to the formation of active oxygen species, while there was no fluorescence in the cortical cells. There was a higher Ca concentration in the cells walls of the endodermis and epidermis, compared to the rest of the S-starved root tissues. A higher pH was observed, mainly in the cell walls of the hypodermis and to a lesser extent in the cell walls of the endodermis. Superoxide anion and hydrogen peroxide was found in degenerating cells of the root cortex. There was no fluorescence of nuclei in the cortex of S-starved roots.	en
heal.journalName	Planta	en
dc.identifier.issue	3	en
dc.identifier.volume	217	en
dc.identifier.doi	10.1007/s00425-003-1007-6	en
dc.identifier.spage	382	en
dc.identifier.epage	391	en