Accumulation of a cocoa-butter-like lipid by Yarrowia lipolytica cultivated on agro-industrial residues

dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Muniglia, L	en
dc.contributor.author	Chevalot, I	en
dc.contributor.author	Aggelis, G	en
dc.contributor.author	Marc, I	en
dc.date.accessioned	2014-06-06T06:45:25Z
dc.date.available	2014-06-06T06:45:25Z
dc.date.issued	2003	en
dc.identifier.issn	03438651	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00284-002-3833-3	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2432
dc.subject.other	citric acid	en
dc.subject.other	glucose	en
dc.subject.other	glycerol	en
dc.subject.other	lauric acid	en
dc.subject.other	linoleic acid	en
dc.subject.other	myristic acid	en
dc.subject.other	oleic acid	en
dc.subject.other	palmitic acid	en
dc.subject.other	saturated fatty acid	en
dc.subject.other	stearic acid	en
dc.subject.other	Theobroma oil	en
dc.subject.other	tristearin	en
dc.subject.other	citric acid	en
dc.subject.other	fatty acid	en
dc.subject.other	stearic acid	en
dc.subject.other	stearic acid derivative	en
dc.subject.other	Theobroma oil	en
dc.subject.other	article	en
dc.subject.other	culture medium	en
dc.subject.other	fungus culture	en
dc.subject.other	fungus growth	en
dc.subject.other	lipid composition	en
dc.subject.other	lipid storage	en
dc.subject.other	lipogenesis	en
dc.subject.other	microbial metabolism	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.subject.other	Yarrowia lipolytica	en
dc.subject.other	fat intake	en
dc.subject.other	growth, development and aging	en
dc.subject.other	lipid metabolism	en
dc.subject.other	metabolism	en
dc.subject.other	Yarrowia	en
dc.subject.other	Animalia	en
dc.subject.other	Fungi	en
dc.subject.other	Theobroma	en
dc.subject.other	Theobroma cacao	en
dc.subject.other	Yarrowia lipolytica	en
dc.subject.other	Citric Acid	en
dc.subject.other	Culture Media	en
dc.subject.other	Dietary Fats	en
dc.subject.other	Fatty Acids	en
dc.subject.other	Glucose	en
dc.subject.other	Glycerol	en
dc.subject.other	Lipid Metabolism	en
dc.subject.other	Stearic Acids	en
dc.subject.other	Yarrowia	en
dc.title	Accumulation of a cocoa-butter-like lipid by Yarrowia lipolytica cultivated on agro-industrial residues	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00284-002-3833-3	en
heal.publicationDate	2003	en
heal.abstract	Yarrowia lipolytica was cultivated on mixtures of saturated free fatty acids (an industrial derivative of animal fat called stearin), technical glycerol (the main by-product of bio-diesel production facilities), and glucose. The utilization of technical glycerol and stearin as co-substrates resulted in higher lipid synthesis and increased citric acid production than the combination of glucose and stearin. The lipids produced contained significant amounts of stearic acid (50-70%, wt/wt) and lower ones of palmitic (15-20%, wt/wt), oleic (7-20%, wt/wt), and linoleic (2-7%, wt/wt) acid. Single-cell oil having a composition similar to cocoa-butter up to 3.4 g/L was produced, whereas in some cases relatively increased citric acid quantities (up to 14 g/L) were excreted into the growth medium. The microorganism presented a high specificity for lauric, myristic, and palmitic acid, while a discrimination for the stearic acid was observed. As a conclusion, microbial metabolism could be directed by using mixtures of inexpensive saturated fats, glycerol, and glucose as co-substrates, in order to accumulate lipids with predetermined composition, e.g., cocoa-butter equivalents.	en
heal.journalName	Current Microbiology	en
dc.identifier.issue	2	en
dc.identifier.volume	46	en
dc.identifier.doi	10.1007/s00284-002-3833-3	en
dc.identifier.spage	124	en
dc.identifier.epage	130	en