Influence of in vitro culture medium on Epidendrum radicans seed germination, seedling growth and ex vitro establishment

dc.contributor.author	Pateli, P	en
dc.contributor.author	Papafotiou, M	en
dc.contributor.author	Chronopoulos, J	en
dc.date.accessioned	2014-06-06T06:45:15Z
dc.date.available	2014-06-06T06:45:15Z
dc.date.issued	2003	en
dc.identifier.issn	05677572	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2324
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-45749126518&partnerID=40&md5=ce01d8384b6eddf9d92f37c168b09f8e	en
dc.subject	Acclimatization	en
dc.subject	Activated charcoal	en
dc.subject	Epidendrum ibaguense	en
dc.subject	Micropropagation	en
dc.subject	Orchid	en
dc.subject	Sowing density	en
dc.title	Influence of in vitro culture medium on Epidendrum radicans seed germination, seedling growth and ex vitro establishment	en
heal.type	conferenceItem	en
heal.publicationDate	2003	en
heal.abstract	In vitro propagation of Epidendrum radicans (E. ibaguense) by seed, and the ability for ex vitro acclimatization and establishment of the seedlings was studied in this work. Liquid and agar-solidified (8 g l-1) MS and VW media enriched with 1 mg l-1 NAA, 1 mg l-1 BA and 15% coconut milk were compared. Two experiments were carried out, one with sparse (50 seeds/vase) and another with dense (∼250 seeds/vase) sowing. Liquid media compared to solid gave much longer seedlings, as well as higher percentage of protocorm like bodies (PLBs) and branched seedlings. High percentage of the responded seeds formed PLBs in liquid VW. In the case of dense plating, liquid MS gave more vigorous seedlings and the seedlings grown on liquid MS were established ex vitro at a much higher percentage. After germination, activated charcoal addition (2 g l-1) to the growing medium increased ex vitro establishment of those seedlings grown on liquid and solid VW and on solid MS medium.	en
heal.journalName	Acta Horticulturae	en
dc.identifier.volume	616	en
dc.identifier.spage	189	en
dc.identifier.epage	192	en