| heal.abstract |
Lilium chalcedonicum bulblets were regenerated on bulb-scale explants cultured on MS salts supplemented with 0.04 mg l-1 thiamine, 100 mg l-1 myoinositol, 30 g l-1 sucrose and 0.5 mg l -1 2,4-D and BA, at 25°C and 16h photoperiod of fluorescent light at about 37.5 μmol m-2 s-1. After 80 days of culture on the initiation medium, the regenerated bulblets were excised and transferred onto plain (without plant growth regulators) medium, kept for 30 or 60 days under the same environmental conditions and then moved to 4°C and continuous darkness, where they remained for 120 or 90 days, respectively. Eighty days after the beginning of culture, 70% of bulblets formed leaflets. Leaflet number did not change during cold storage, while 88% of the bulblets sprouted and 90% of them rooted during this period. After cold storage, bigger than 3 mm in diameter bulblets were transferred ex vitro on peat: perlite (1:1, by volume) under intermittent mist. Bulblets that remained for 60 days on plain medium at 25°C and 16h photoperiod followed by 90 days at 4°C and continuous darkness grew bigger in diameter and acclimatized (appearance of new leaf) 15 days earlier than those remained for 30 days at 25°C and 16h photoperiod and 120 days at 4°C and continuous darkness. Also, bulblets that bore leaflets acclimatized faster, but they had lower development rate during the following one-month period compared to bulblets without leaflets. |
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