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Discrimination and genetic diversity among cultivated olives of Greece using RAPD markers

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dc.contributor.author Nikoloudakis, N en
dc.contributor.author Banilas, G en
dc.contributor.author Gazis, F en
dc.contributor.author Hatzopoulos, P en
dc.contributor.author Metzidakis, J en
dc.date.accessioned 2014-06-06T06:45:15Z
dc.date.available 2014-06-06T06:45:15Z
dc.date.issued 2003 en
dc.identifier.issn 00031062 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2318
dc.relation.uri http://www.scopus.com/inward/record.url?eid=2-s2.0-0042123698&partnerID=40&md5=eff666297fdb57230f8bfa3026e20621 en
dc.subject Cultivars en
dc.subject Diversity en
dc.subject Olea europaea en
dc.subject Polymorphism en
dc.subject.other Embryophyta en
dc.subject.other Olea europaea en
dc.subject.other Oleaceae en
dc.title Discrimination and genetic diversity among cultivated olives of Greece using RAPD markers en
heal.type journalArticle en
heal.publicationDate 2003 en
heal.abstract Random amplified polymorphic DNA (RAPD) markers were used to study the genetic diversity and to discriminate among 33 Greek olive (Olea europaea L.) cultivars. Three feral forms from Crete and five foreign cultivars recently introduced into Greece were also included. Nineteen primers were selected which produced 64 reproducible polymorphic bands in the 41 olive genotypes studied, with an average of 3.4 informative markers per primer. The RAPD markers resulted in 135 distinct electrophoretic patterns, with an average of 7.1 patterns per primer. Based on either unique or combined patterns, all genotypes could be identified. Genetic similarities between genotypes were estimated using the Dice similarity index and these indicated that a high degree of diversity exists within the Greek olive germplasm. Using the unweighted pair-group method (UPGMA) most cultivars were clustered into two main groups according to their fruit size or commercial use (table or olive oil). However, poor correlation was detected between clustering of cultivars and their principal area of cultivation. RAPD marker data were subjected to nonmetric multidimentional scaling (NMDS) which produced results similar to those of the UPGMA analysis. The results presented here contribute to a comprehensive understanding of cultivated Greek olive germplasm and provide information that could be important for cultural purposes and breeding programs. en
heal.journalName Journal of the American Society for Horticultural Science en
dc.identifier.issue 5 en
dc.identifier.volume 128 en
dc.identifier.spage 741 en
dc.identifier.epage 746 en


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