| dc.contributor.author |
Andreou, VG |
en |
| dc.contributor.author |
Clonis, YD |
en |
| dc.date.accessioned |
2014-06-06T06:45:11Z |
|
| dc.date.available |
2014-06-06T06:45:11Z |
|
| dc.date.issued |
2002 |
en |
| dc.identifier.issn |
09565663 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/S0956-5663(01)00261-5 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2291 |
|
| dc.subject |
Biosensor |
en |
| dc.subject |
Bromocresol purple |
en |
| dc.subject |
Carbaryl |
en |
| dc.subject |
Cholinesterase |
en |
| dc.subject |
Dichlorvos |
en |
| dc.subject |
Fiber optics |
en |
| dc.subject |
Inhibition |
en |
| dc.subject |
Sol-gel |
en |
| dc.subject.other |
Enzyme immobilization |
en |
| dc.subject.other |
Fiber optics |
en |
| dc.subject.other |
Pesticides |
en |
| dc.subject.other |
Polymeric membranes |
en |
| dc.subject.other |
Sol-gels |
en |
| dc.subject.other |
Bioactive materials |
en |
| dc.subject.other |
Biosensors |
en |
| dc.subject.other |
bromcresol purple |
en |
| dc.subject.other |
carbaril |
en |
| dc.subject.other |
cholinesterase |
en |
| dc.subject.other |
dichlorvos |
en |
| dc.subject.other |
pesticide |
en |
| dc.subject.other |
polyvinylidene fluoride |
en |
| dc.subject.other |
water |
en |
| dc.subject.other |
accuracy |
en |
| dc.subject.other |
analytic method |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
artificial membrane |
en |
| dc.subject.other |
biosensor |
en |
| dc.subject.other |
calibration |
en |
| dc.subject.other |
enzyme immobilization |
en |
| dc.subject.other |
enzyme inhibition |
en |
| dc.subject.other |
fiber optics |
en |
| dc.subject.other |
gel |
en |
| dc.subject.other |
hydrolysis |
en |
| dc.subject.other |
pH |
en |
| dc.subject.other |
reproducibility |
en |
| dc.subject.other |
signal transduction |
en |
| dc.subject.other |
temperature |
en |
| dc.subject.other |
water sampling |
en |
| dc.subject.other |
Biosensing Techniques |
en |
| dc.subject.other |
Bromcresol Purple |
en |
| dc.subject.other |
Carbaryl |
en |
| dc.subject.other |
Cholinesterases |
en |
| dc.subject.other |
Dichlorvos |
en |
| dc.subject.other |
Enzymes, Immobilized |
en |
| dc.subject.other |
Fiber Optics |
en |
| dc.subject.other |
Gels |
en |
| dc.subject.other |
Insecticides |
en |
| dc.subject.other |
Water |
en |
| dc.title |
A portable fiber-optic pesticide biosensor based on immobilized cholinesterase and sol-gel entrapped bromcresol purple for in-field use |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/S0956-5663(01)00261-5 |
en |
| heal.publicationDate |
2002 |
en |
| heal.abstract |
A fiber-optic biosensor for the detection and determination of the pesticides carbaryl and dichlorvos was developed. The sensing bioactive material was a three-layer sandwich. The enzyme cholinesterase was immobilized on the outer layer, consisting of hydrophilic modified polyvinylidenefluoride membrane. The membrane was in contact with an intermediate sol-gel layer that incorporated bromcresol purple, deposited on an inner glass disk. The sensor operated in a static mode at room temperature and the rate of the inhibited reaction served as an analytical signal. Calibration curves were obtained for carbaryl and dichlorvos, with useful concentration ranges 0.11-8.0 mg l -1 for carbaryl and 5.0-30 μg l -1 for dichlorvos. The respective detection limits were 108 μg l -1 and 5.2 μg l -1. The method reproducibility was in the order of ±3-5%. The method was successfully applied to the detection and determination of these pesticides in real water samples, without sample preparation steps. Recovery experiments were made and the accuracy of the method was 94.9%. No enzyme regeneration steps were applied and the sensor lifetime was 3 weeks (30% activity reduction). The bioactive mini sandwich can easily be replaced by simply unscrewing the terminal holding ring of the probe and placing a new sandwich, before the sensor is ready for use. © 2002 Elsevier Science B.V. All rights reserved. |
en |
| heal.journalName |
Biosensors and Bioelectronics |
en |
| dc.identifier.issue |
1-2 |
en |
| dc.identifier.volume |
17 |
en |
| dc.identifier.doi |
10.1016/S0956-5663(01)00261-5 |
en |
| dc.identifier.spage |
61 |
en |
| dc.identifier.epage |
69 |
en |