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Yarrowia lipolytica as a potential producer of citric acid from raw glycerol

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dc.contributor.author Papanikolaou, S en
dc.contributor.author Muniglia, L en
dc.contributor.author Chevalot, I en
dc.contributor.author Aggelis, G en
dc.contributor.author Marc, I en
dc.date.accessioned 2014-06-06T06:45:11Z
dc.date.available 2014-06-06T06:45:11Z
dc.date.issued 2002 en
dc.identifier.issn 13645072 en
dc.identifier.uri http://dx.doi.org/10.1046/j.1365-2672.2002.01577.x en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2290
dc.subject.other citric acid en
dc.subject.other glucose en
dc.subject.other glycerol en
dc.subject.other lipid en
dc.subject.other nitrogen en
dc.subject.other citric acid en
dc.subject.other microbiology en
dc.subject.other article en
dc.subject.other biosynthesis en
dc.subject.other controlled study en
dc.subject.other extraction en
dc.subject.other fermentation en
dc.subject.other fungal biomass en
dc.subject.other fungus growth en
dc.subject.other growth rate en
dc.subject.other lipid composition en
dc.subject.other lipid storage en
dc.subject.other morphology en
dc.subject.other nonhuman en
dc.subject.other pH en
dc.subject.other species cultivation en
dc.subject.other Yarrowia lipolytica en
dc.subject.other Biomass en
dc.subject.other Citric Acid en
dc.subject.other Culture Media en
dc.subject.other Glucose en
dc.subject.other Glycerol en
dc.subject.other Lipids en
dc.subject.other Yarrowia en
dc.subject.other Fungi en
dc.subject.other Yarrowia lipolytica en
dc.title Yarrowia lipolytica as a potential producer of citric acid from raw glycerol en
heal.type journalArticle en
heal.identifier.primary 10.1046/j.1365-2672.2002.01577.x en
heal.publicationDate 2002 en
heal.abstract Aims: To study the biochemical response of Yarrowia lipolytica LGAM S(7)1 during growth on raw glycerol (the main by-product of bio-diesel production units) in order to produce metabolic products of industrial significance. Methods and Results: Yarrowia lipolytica was cultivated on raw glycerol or glucose in flasks. Although nitrogen-limited media were employed, growth was not followed by production of reserve lipid. Nitrogen limitation led to citric acid excretion. Growth and citric acid production parameters on glycerol were similar to those obtained on glucose. When high initial glycerol media were used, citric acid up to 35 g l-1 (yield 0.42-0.44 g acid g-1 glycerol consumed) was produced. Conclusions: Raw glycerol was an adequate substrate for Y. lipolytica. Growth was not followed by reserve lipid accumulation, but amounts of citric acid were produced. Significance and Impact of the Study: Raw glycerol is an industrial feedstock appearing in increasing quantities as the main by-product of bio-diesel production facilities. The present study describes an alternative way of glycerol valorization, with the production of remarkable amounts of citric acid, in addition to its main valorization way (production of 1,3-propanediol by bacteria). en
heal.journalName Journal of Applied Microbiology en
dc.identifier.issue 4 en
dc.identifier.volume 92 en
dc.identifier.doi 10.1046/j.1365-2672.2002.01577.x en
dc.identifier.spage 737 en
dc.identifier.epage 744 en


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