Spectroimmunochemistry using colloidal gold bioconjugates

dc.contributor.author	Kamnev, AA	en
dc.contributor.author	Dykman, LA	en
dc.contributor.author	Tarantilis, PA	en
dc.contributor.author	Polissiou, MG	en
dc.date.accessioned	2014-06-06T06:45:09Z
dc.date.available	2014-06-06T06:45:09Z
dc.date.issued	2002	en
dc.identifier.issn	01448463	en
dc.identifier.uri	http://dx.doi.org/10.1023/A:1022077920527	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2269
dc.subject	Colloidal gold bioconjugates	en
dc.subject	Fourier transform infrared spectroscopy (FTIR)	en
dc.subject	Human immunoglobulin G	en
dc.subject	Protein A	en
dc.subject	Spectroimmunochemistry	en
dc.subject	Surface-enhanced infrared absorption (SEIRA)	en
dc.subject.other	colloidal gold	en
dc.subject.other	immunoglobulin G	en
dc.subject.other	protein A	en
dc.subject.other	absorption spectroscopy	en
dc.subject.other	article	en
dc.subject.other	immunochemistry	en
dc.subject.other	infrared spectrometry	en
dc.subject.other	protein interaction	en
dc.subject.other	quality control	en
dc.subject.other	spectroimmunochemistry	en
dc.subject.other	surface enhanced infrared absorption spectroscopy	en
dc.subject.other	technique	en
dc.subject.other	Binding Sites	en
dc.subject.other	Gold Colloid	en
dc.subject.other	Humans	en
dc.subject.other	Immunoglobulin G	en
dc.subject.other	Immunohistochemistry	en
dc.subject.other	Molecular Conformation	en
dc.subject.other	Reproducibility of Results	en
dc.subject.other	Spectroscopy, Fourier Transform Infrared	en
dc.subject.other	Staphylococcal Protein A	en
dc.subject.other	Seira	en
dc.title	Spectroimmunochemistry using colloidal gold bioconjugates	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1022077920527	en
heal.publicationDate	2002	en
heal.abstract	Using surface-enhanced infrared absorption (SEIRA) spectroscopy of dry films of colloidal gold (CG) bioconjugates with protein A, it is shown that certain characteristic bands of the protein (e.g., amide I, amide II and some other vibration modes) are essentially affected by the metal surface. Thus, the method may be used for controlling the quality of such bioconjugates. Moreover, it is demonstrated that the biospecific reaction of protein A attached to CG particles with human immunoglobulin G (IgG) results in further essential changes in SEIRA spectra, providing a means for an easy and rapid IR spectroscopic detection of biospecific immunochemical interactions (i.e., spectroimmunochemistry). The results obtained can form a basis for developing test systems for detecting various biospecific interactions.	en
heal.journalName	Bioscience Reports	en
dc.identifier.issue	5-6	en
dc.identifier.volume	22	en
dc.identifier.doi	10.1023/A:1022077920527	en
dc.identifier.spage	541	en
dc.identifier.epage	547	en