Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly Bactrocera oleae

dc.contributor.author	Vontas, JG	en
dc.contributor.author	Hejazi, MJ	en
dc.contributor.author	Hawkes, NJ	en
dc.contributor.author	Cosmidis, N	en
dc.contributor.author	Loukas, M	en
dc.contributor.author	Hemingway, J	en
dc.date.accessioned	2014-06-06T06:45:08Z
dc.date.available	2014-06-06T06:45:08Z
dc.date.issued	2002	en
dc.identifier.issn	09621075	en
dc.identifier.uri	http://dx.doi.org/10.1046/j.1365-2583.2002.00343.x	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2265
dc.subject	AChE cDNA	en
dc.subject	Bactrocera oleae	en
dc.subject	Insecticide resistance	en
dc.subject	Mutations	en
dc.subject.other	Bactrocera oleae	en
dc.subject.other	Drosophila melanogaster	en
dc.subject.other	Hexapoda	en
dc.subject.other	Insecta	en
dc.subject.other	Torpedo	en
dc.subject.other	Torpedo californica	en
dc.subject.other	acetylcholinesterase	en
dc.subject.other	cholinesterase inhibitor	en
dc.subject.other	dimethoate	en
dc.subject.other	drug derivative	en
dc.subject.other	insecticide	en
dc.subject.other	omethoate	en
dc.subject.other	amino acid sequence	en
dc.subject.other	animal	en
dc.subject.other	article	en
dc.subject.other	chemical structure	en
dc.subject.other	comparative study	en
dc.subject.other	DNA sequence	en
dc.subject.other	enzymology	en
dc.subject.other	fly	en
dc.subject.other	genetics	en
dc.subject.other	insecticide resistance	en
dc.subject.other	metabolism	en
dc.subject.other	molecular genetics	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	point mutation	en
dc.subject.other	protein conformation	en
dc.subject.other	sequence homology	en
dc.subject.other	Southern blotting	en
dc.subject.other	Acetylcholinesterase	en
dc.subject.other	Amino Acid Sequence	en
dc.subject.other	Animals	en
dc.subject.other	Base Sequence	en
dc.subject.other	Blotting, Southern	en
dc.subject.other	Cholinesterase Inhibitors	en
dc.subject.other	Dimethoate	en
dc.subject.other	Diptera	en
dc.subject.other	Insecticide Resistance	en
dc.subject.other	Insecticides	en
dc.subject.other	Models, Molecular	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Point Mutation	en
dc.subject.other	Protein Conformation	en
dc.subject.other	Sequence Analysis, DNA	en
dc.subject.other	Sequence Homology, Amino Acid	en
dc.title	Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly Bactrocera oleae	en
heal.type	journalArticle	en
heal.identifier.primary	10.1046/j.1365-2583.2002.00343.x	en
heal.publicationDate	2002	en
heal.abstract	A 2.2-kb full length cDNA containing an ORF encoding a putative acetylcholinesterase (AChE) precursor of 673 amino acid residues was obtained by a combined degenerate PCR and RACE strategy from an organophosphate-susceptible Bactrocera oleae strain. A comparison of cDNA sequences of individual insects from susceptible and resistant strains, coupled with an enzyme inhibition assay with omethoate, indicated a novel glycine-serine substitution (G488S), at an amino acid residue which is highly conserved across species (G396 of Torpedo californica AChE), as a likely cause of AChE insensitivity. This mutation was also associated with a 35-40% reduction in AChE catalytic efficiency. The 1199V substitution, which confers low levels of resistance in Drosophila, was also present in B. oleae (1214V) and in combination with G488S produced up to a 16-fold decrease in insecticide sensitivity. This is the first agricultural pest where resistance has been associated with an alteration in AChE, which arises from point mutations located within the active site gorge of the enzyme.	en
heal.journalName	Insect Molecular Biology	en
dc.identifier.issue	4	en
dc.identifier.volume	11	en
dc.identifier.doi	10.1046/j.1365-2583.2002.00343.x	en
dc.identifier.spage	329	en
dc.identifier.epage	336	en