| dc.contributor.author |
Andreou, VG |
en |
| dc.contributor.author |
Clonis, YD |
en |
| dc.date.accessioned |
2014-06-06T06:45:06Z |
|
| dc.date.available |
2014-06-06T06:45:06Z |
|
| dc.date.issued |
2002 |
en |
| dc.identifier.issn |
00032670 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/S0003-2670(02)00250-7 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2253 |
|
| dc.subject |
Atrazine |
en |
| dc.subject |
Biosensor |
en |
| dc.subject |
Bromcresol green |
en |
| dc.subject |
Fiber optics |
en |
| dc.subject |
Glutathione S-transferase |
en |
| dc.subject |
Sol-gel |
en |
| dc.subject.other |
alachlor |
en |
| dc.subject.other |
atrazine |
en |
| dc.subject.other |
bromocresol green |
en |
| dc.subject.other |
carbaril |
en |
| dc.subject.other |
glutathione transferase |
en |
| dc.subject.other |
herbicide |
en |
| dc.subject.other |
isoenzyme |
en |
| dc.subject.other |
pesticide |
en |
| dc.subject.other |
polyvinylidene fluoride |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
calibration |
en |
| dc.subject.other |
concentration (parameters) |
en |
| dc.subject.other |
enzyme activity |
en |
| dc.subject.other |
enzyme assay |
en |
| dc.subject.other |
enzyme immobilization |
en |
| dc.subject.other |
enzyme mechanism |
en |
| dc.subject.other |
enzyme substrate |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
fiber optic biosensor |
en |
| dc.subject.other |
gel |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
protein expression |
en |
| dc.subject.other |
reproducibility |
en |
| dc.subject.other |
temperature |
en |
| dc.subject.other |
water sampling |
en |
| dc.subject.other |
Zea mays |
en |
| dc.title |
Novel fiber-optic biosensor based on immobilized glutathione S-transferase and sol-gel entrapped bromcresol green for the determination of atrazine |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/S0003-2670(02)00250-7 |
en |
| heal.publicationDate |
2002 |
en |
| heal.abstract |
The aim of this work was to investigate, for the first time, the potential of the enzyme glutathione S-transferase I (isoenzyme GST-I) for uses in analytical chemistry. A novel fiber-optic biosensor for the detection and determination of the triazine herbicide atrazine was developed based on maize GST-I expressed in E. coli. The sensing bioactive material was a three-layer mini-sandwich. The enzyme was immobilized on the outer layer that consisted of a hydrophilic polyvinylidenefluoride membrane. This membrane was supported on an inner glass disk by means of an intermediate binder sol-gel layer that incorporated bromcresol green (BCG). The biosensor operated in a static mode at 25°C and the rate of the enzymatic reaction, using atrazine as a substrate, served as an analytical signal. A calibration curve was obtained for atrazine, with analytically useful concentration range 2.52-125μM. The sensor detection limit was 0.84μM. The reproducibility of atrazine sensing was in the order of ±3-5%. The method was successfully applied to the determination of this herbicide in real water samples, without sample preparation steps. Atrazine recovery ranged between 85 and 110%. No interference from other pesticides, such as alachlor and carbaryl was observed in the absence of atrazine. The immobilized enzyme retained about 75% of its original activity after 1 month use. Simply unscrewing the terminal holding ring of the probe and placing a new bioactive sandwich could easily replace a deteriorated mini-sandwich. © 2002 Elsevier Science B.V. All rights reserved. |
en |
| heal.journalName |
Analytica Chimica Acta |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
460 |
en |
| dc.identifier.doi |
10.1016/S0003-2670(02)00250-7 |
en |
| dc.identifier.spage |
151 |
en |
| dc.identifier.epage |
161 |
en |