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Microbial colonization of naturally black olives during fermentation and associated biochemical activities in the cover brine

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dc.contributor.author Nychas, G-JE en
dc.contributor.author Panagou, EZ en
dc.contributor.author Parker, ML en
dc.contributor.author Waldron, KW en
dc.contributor.author Tassou, CC en
dc.date.accessioned 2014-06-06T06:45:06Z
dc.date.available 2014-06-06T06:45:06Z
dc.date.issued 2002 en
dc.identifier.issn 02668254 en
dc.identifier.uri http://dx.doi.org/10.1046/j.1472-765x.2002.01077.x en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2246
dc.subject.other citric acid en
dc.subject.other malic acid en
dc.subject.other tartaric acid en
dc.subject.other microorganism en
dc.subject.other article en
dc.subject.other bacterial colonization en
dc.subject.other biofilm en
dc.subject.other controlled study en
dc.subject.other Enterobacteriaceae en
dc.subject.other fermentation en
dc.subject.other food processing en
dc.subject.other lactic acid bacterium en
dc.subject.other microbial growth en
dc.subject.other molecular dynamics en
dc.subject.other nonhuman en
dc.subject.other olive en
dc.subject.other pH en
dc.subject.other Pseudomonas en
dc.subject.other scanning electron microscopy en
dc.subject.other yeast en
dc.subject.other Biofilms en
dc.subject.other Fermentation en
dc.subject.other Food Microbiology en
dc.subject.other Fruit en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Lactic Acid en
dc.subject.other Salts en
dc.subject.other Yeasts en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Enterobacteriaceae en
dc.subject.other Oleaceae en
dc.subject.other Pseudomonas en
dc.title Microbial colonization of naturally black olives during fermentation and associated biochemical activities in the cover brine en
heal.type journalArticle en
heal.identifier.primary 10.1046/j.1472-765x.2002.01077.x en
heal.publicationDate 2002 en
heal.abstract Aims: To establish the site of microbial growth on naturally black fermented table olives, and to monitor the population dynamics of yeasts and selected micro-organisms together with the changes in organic acid profile and pH in the cover brine during fermentation. Methods and Results: During fermentation, the numbers of Enterobacteriaceae and Pseudomonas spp. in the brine decreased whilst lactic acid bacteria and yeast populations increased. Scanning electron microscopy showed that a yeast-rich biofilm developed on the epicuticular wax of the olive skin during fermentation. Yeasts also predominated in the stomatal openings, but bacteria were more numerous in intercellular spaces in the sub-stomatal flesh. Citric, malic and tartaric acids were the major organic acids accumulating in the brine during fermentation. Conclusions: Micro-organisms associated with the skin, stomata and flesh in fermenting black olives may experience different local conditions to those prevailing in the cover brine. Significant and Impact of the Study: These are the first observations of the micro-organisms associated with the fruit of naturally fermented black olives and of the accumulation of specific organic acids during fermentation. en
heal.journalName Letters in Applied Microbiology en
dc.identifier.issue 3 en
dc.identifier.volume 34 en
dc.identifier.doi 10.1046/j.1472-765x.2002.01077.x en
dc.identifier.spage 173 en
dc.identifier.epage 177 en


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