In vitro propagation of olive (Olea europaea L.) cv. Koroneiki

dc.contributor.author	Roussos, PA	en
dc.contributor.author	Pontikis, CA	en
dc.date.accessioned	2014-06-06T06:45:04Z
dc.date.available	2014-06-06T06:45:04Z
dc.date.issued	2002	en
dc.identifier.issn	01676903	en
dc.identifier.uri	http://dx.doi.org/10.1023/A:1020824330589	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2234
dc.subject	In vitro culture	en
dc.subject	Olive	en
dc.subject	Olive knot extract	en
dc.subject	Pseudomonas savastanoi pv. savastanoi	en
dc.subject	Rooting	en
dc.subject.other	Forestry	en
dc.subject.other	Growth kinetics	en
dc.subject.other	Survival rates	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Juglans	en
dc.subject.other	Olea europaea	en
dc.subject.other	Pseudomonas	en
dc.subject.other	Pseudomonas savastanoi	en
dc.title	In vitro propagation of olive (Olea europaea L.) cv. Koroneiki	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1020824330589	en
heal.publicationDate	2002	en
heal.abstract	Single node explants of 'Koroneiki' olive trees were cultured for one month on a modified Driver-Kuniyuki for Walnut medium, lacking growth regulators. The explants were subcultured once a month on a medium supplemented with zeatin riboside, 6-(-γ-γ-dimethylallylamino) purine, 6-benzyladenine or thidiazuron. Zeatin riboside proved to be superior to other cytokinins in inducing shoot proliferation. The combination of olive knot extract at 25 or 50 mg 1 -1 with cytokinins suppressed shoot proliferation. After two months at the proliferation stage, the explants were cultured for one week in the dark in 1 ml liquid Woody Plant Medium supplemented with IBA, α-NAA or IBA+α-NAA. The explants were then transferred to the same solid medium lacking growth regulators, with a small layer of perlite on the surface. The combination of the two auxins at 1+1 mg 1 -1 resulted in almost 76% rooting. The combination of olive knot extract at 50 mg 1 -1 with auxins increased the rooting percentage up to almost 87%. Artificial infection of explants with the bacterium Pseudomonas savastanoi pv. savastanoi inhibited rhizogenesis, even in the presence of auxins. Rooted explants were successfully acclimatised under a mist system, with the survival rate reaching almost 75%.	en
heal.journalName	Plant Growth Regulation	en
dc.identifier.issue	3	en
dc.identifier.volume	37	en
dc.identifier.doi	10.1023/A:1020824330589	en
dc.identifier.spage	295	en
dc.identifier.epage	304	en