Growth regulator pretreatment improves somatic embryogenesis from leaves of squash (Cucurbita pepo I.) and melon (Cucumis melo I.)

dc.contributor.author	Kintzios, S	en
dc.contributor.author	Sereti, E	en
dc.contributor.author	Bluchos, P	en
dc.contributor.author	Drossopoulos, JB	en
dc.contributor.author	Kitsaki, CK	en
dc.contributor.author	Liopa-Tsakalidis, A	en
dc.date.accessioned	2014-06-06T06:45:04Z
dc.date.available	2014-06-06T06:45:04Z
dc.date.issued	2002	en
dc.identifier.issn	07217714	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00299-002-0448-x	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2231
dc.subject	Callus culture	en
dc.subject	Embryo germination	en
dc.subject	Leaf explant	en
dc.subject	Proembryo induction	en
dc.subject.other	Organic acids	en
dc.subject.other	Plant cell culture	en
dc.subject.other	Growth regulators	en
dc.subject.other	Somatic embryogenesis	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Cucumis	en
dc.subject.other	Cucumis melo	en
dc.subject.other	Cucurbita	en
dc.subject.other	Cucurbita pepo	en
dc.subject.other	Pepo	en
dc.subject.other	Torpedo	en
dc.title	Growth regulator pretreatment improves somatic embryogenesis from leaves of squash (Cucurbita pepo I.) and melon (Cucumis melo I.)	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00299-002-0448-x	en
heal.publicationDate	2002	en
heal.abstract	Leaf explants of squash (Cucurbita pepo L.) and melon (Cucumis melo L.) were pretreated initially with 113.1, 226.2 or 452.4 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 46.5, 93 or 186 μM kinetin or a combination of both at the above concentrations, for 6, 24 or 48 h. After pretreatment, explants were transferred to an agar-solidified medium that was not supplemented with growth regulators or to a species-specific standard induction medium. Control explants from each species were incubated directly on the species-specific standard induction medium. Initial pretreatment of squash explants with 186 μM kinetin and of melon explants with 226.2 μM 2,4-D for 48 h significantly promoted the formation of somatic embryos which developed further to the torpedo-shape stage and germinated. Under these conditions at least four plants can be regenerated per square centimeter of explant surface, thus achieving an increase over non-pretreated cultures of 143% and 130% for squash and melon, respectively.	en
heal.journalName	Plant Cell Reports	en
dc.identifier.issue	1	en
dc.identifier.volume	21	en
dc.identifier.doi	10.1007/s00299-002-0448-x	en
dc.identifier.spage	1	en
dc.identifier.epage	8	en