| dc.contributor.author |
Politis, I |
en |
| dc.contributor.author |
Zavizion, B |
en |
| dc.contributor.author |
Cheli, F |
en |
| dc.contributor.author |
Baldi, A |
en |
| dc.date.accessioned |
2014-06-06T06:45:02Z |
|
| dc.date.available |
2014-06-06T06:45:02Z |
|
| dc.date.issued |
2002 |
en |
| dc.identifier.issn |
00220299 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1017/S0022029902005502 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2220 |
|
| dc.subject |
Bovine neutrophils |
en |
| dc.subject |
Urokinase receptor |
en |
| dc.subject.other |
Animal |
en |
| dc.subject.other |
Binding, Competitive |
en |
| dc.subject.other |
Blotting, Northern |
en |
| dc.subject.other |
Carcinogens |
en |
| dc.subject.other |
Cattle |
en |
| dc.subject.other |
Female |
en |
| dc.subject.other |
Neutrophils |
en |
| dc.subject.other |
Plasmin |
en |
| dc.subject.other |
Protein Kinase C |
en |
| dc.subject.other |
Receptors, Cell Surface |
en |
| dc.subject.other |
RNA, Messenger |
en |
| dc.subject.other |
Tetradecanoylphorbol Acetate |
en |
| dc.subject.other |
Tissue Plasminogen Activator |
en |
| dc.subject.other |
Up-Regulation |
en |
| dc.subject.other |
Urinary Plasminogen Activator |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Bovinae |
en |
| dc.subject.other |
Rickettsia sp. PAR |
en |
| dc.subject.other |
carcinogen |
en |
| dc.subject.other |
cell surface receptor |
en |
| dc.subject.other |
messenger RNA |
en |
| dc.subject.other |
phorbol 13 acetate 12 myristate |
en |
| dc.subject.other |
plasmin |
en |
| dc.subject.other |
plasminogen activator, urokinase receptors |
en |
| dc.subject.other |
protein kinase C |
en |
| dc.subject.other |
tissue plasminogen activator |
en |
| dc.subject.other |
urokinase |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
animal disease |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
binding competition |
en |
| dc.subject.other |
cattle |
en |
| dc.subject.other |
drug effect |
en |
| dc.subject.other |
female |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
neutrophil |
en |
| dc.subject.other |
Northern blotting |
en |
| dc.subject.other |
physiology |
en |
| dc.subject.other |
upregulation |
en |
| dc.title |
Expression of urokinase plasminogen activator receptor in resting and activated bovine neutrophils |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1017/S0022029902005502 |
en |
| heal.publicationDate |
2002 |
en |
| heal.abstract |
Changes in urokinase-plasminogen activator (u-PA) and u-PA receptor (u-PAR) expression at the protein and mRNA level in resting neutrophils and in neutrophils activated by phorbol myristate acetate (PMA) were examined. Low amounts of u-PA were found intracellularly or membrane-bound in resting neutrophils. However, incubation of resting neutrophils with purified exogenous u-PA (10 IU/ml) revealed extensive binding of u-PA to cell membranes. Excess amino-terminal fragment of the u-PA molecule, a proteolytically inactive fragment of u-PA (amino acids 1-135) blocked binding of exogenous u-PA to the cell membrane, These results, collectively, indicate that the binding of u-PA is specific and that resting neutrophils have unoccupied u-PA receptors on their cell membrane. Addition of PMA led to an increase (P < 0.01) in total cell-associated, membrane-bound u-PA activity and u-PA mRNA expression by bovine neutrophils. In contrast, PMA increased u-PAR mRNA levels but this was accompanied by a decrease (2.5-fold; P < 0.01) in free, unoccupied u-PA binding sites. No significant effects on total cell-associated or membrane-bound u-PA were found when neutrophils were treated with 4-phorbol 12,13 didecanoate, a phorbol ester that does not activate protein kinase C (PKC). Furthermore addition of 1-(5-isoquinolinesylphonyl)-2-methylpiperazine dihydrochloride (H-7), a potent PKC inhibitor, blocked the effect of PMA on total cell-associated u-PA activity. Thus, PKC plays a role in the modulation of u-PA and u-PAR by PMA in bovine neutrophils. |
en |
| heal.journalName |
Journal of Dairy Research |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
69 |
en |
| dc.identifier.doi |
10.1017/S0022029902005502 |
en |
| dc.identifier.spage |
195 |
en |
| dc.identifier.epage |
204 |
en |