Evaluation of white-rot fungi for detoxification and decolorization of effluents from the green olive debittering process

dc.contributor.author	Aggelis, G	en
dc.contributor.author	Ehaliotis, C	en
dc.contributor.author	Nerud, F	en
dc.contributor.author	Stoychev, I	en
dc.contributor.author	Lyberatos, G	en
dc.contributor.author	Zervakis, G	en
dc.date.accessioned	2014-06-06T06:45:02Z
dc.date.available	2014-06-06T06:45:02Z
dc.date.issued	2002	en
dc.identifier.issn	01757598	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00253-002-1005-9	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2217
dc.subject.other	alcohol oxidase	en
dc.subject.other	fungal enzyme	en
dc.subject.other	laccase	en
dc.subject.other	lignin peroxidase	en
dc.subject.other	manganese	en
dc.subject.other	manganese peroxidase	en
dc.subject.other	oxidoreductase	en
dc.subject.other	oxygen	en
dc.subject.other	polyphenol derivative	en
dc.subject.other	quinine derivative	en
dc.subject.other	agricultural emission	en
dc.subject.other	bioremediation	en
dc.subject.other	effluent	en
dc.subject.other	fungus	en
dc.subject.other	alkalinity	en
dc.subject.other	article	en
dc.subject.other	controlled study	en
dc.subject.other	correlation function	en
dc.subject.other	decolorization	en
dc.subject.other	detoxification	en
dc.subject.other	effluent	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme purification	en
dc.subject.other	fungus growth	en
dc.subject.other	nonhuman	en
dc.subject.other	olive	en
dc.subject.other	oxygen consumption	en
dc.subject.other	Phanerochaete	en
dc.subject.other	phytotoxicity	en
dc.subject.other	polymerization	en
dc.subject.other	precipitation	en
dc.subject.other	reduction	en
dc.subject.other	Alcohol Oxidoreductases	en
dc.subject.other	Biodegradation, Environmental	en
dc.subject.other	Color	en
dc.subject.other	Fungi	en
dc.subject.other	Industrial Waste	en
dc.subject.other	Peroxidases	en
dc.subject.other	Plant Oils	en
dc.subject.other	Waste Disposal, Fluid	en
dc.subject.other	Abortiporus biennis	en
dc.subject.other	Cinchona pubescens	en
dc.subject.other	Dichomitus	en
dc.subject.other	Dichomitus squalens	en
dc.subject.other	Fungi	en
dc.subject.other	Inonotus	en
dc.subject.other	Inonotus hispidus	en
dc.subject.other	Irpex	en
dc.subject.other	Irpex lacteus	en
dc.subject.other	Lentinus	en
dc.subject.other	Lentinus tigrinus	en
dc.subject.other	Oleaceae	en
dc.subject.other	Panellus stypticus	en
dc.subject.other	Phanerochaete	en
dc.subject.other	Pleurotus	en
dc.subject.other	Pleurotus ostreatus	en
dc.subject.other	Polyporus	en
dc.subject.other	Trametes	en
dc.subject.other	Trametes hirsuta	en
dc.title	Evaluation of white-rot fungi for detoxification and decolorization of effluents from the green olive debittering process	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00253-002-1005-9	en
heal.publicationDate	2002	en
heal.abstract	Wastewater produced by the debittering process of green olives (GOW) is rich in polyphenolics and presents high chemical oxygen demand and alkalinity values. Eight white-rot fungi (Abortiporus biennis, Dichomitus squalens, Inonotus hispidus, Irpex lacteus, Lentinus tigrinus, Panellus stipticus, Pleurotus ostreatus and Trametes hirsuta) were grown in GOW for 1 month and the reduction in total phenolics, the decolorization activity and the related enzyme activities were compared. Phenolics were efficiently reduced by P. ostreatus (52%) andA. biennis (55%), followed by P. stipticus (42%) and D. squalens (36%), but only P. ostreatus had high decolorization efficiency (49%). Laccase activity was the highest in all of the fungi, followed by manganese-independent peroxidase (MnIP). Substantial manganese peroxidase (MnP) activity was observed only in GOW treated with P. ostreatus and A. biennis, whereas lignin peroxidase (LiP) and veratryl alcohol oxidase (VAOx) activities were not detected. Early measurements of laccase activity were highly correlated (r2=0.91) with the final reduction of total phenolics and could serve as an early indicator of the potential of white-rot fungi to efficiently reduce the amount of total phenolics in GOW. The presence of MnP was, however, required to achieve efficient decolorization. Phytotoxicity of GOW treated with a selected P. ostreatus strain did not decline despite large reductions of the phenolic content (76%). Similarly, in GOW treated with purified laccase from Polyporus pensitius, a reduction in total phenolics which exceeded 50% was achieved; however, it was not accompanied by a decline in phytotoxicity. These results are probably related to the formation of phenoxy radicals and quinonoids, which re-polymerize in the absence of VAOx but do not lead to polymer precipitation in the treated GOW.	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.issue	2-3	en
dc.identifier.volume	59	en
dc.identifier.doi	10.1007/s00253-002-1005-9	en
dc.identifier.spage	353	en
dc.identifier.epage	360	en