Effect of acetone feeding on alcohol dehydrogenase activity in the olive fruit fly, Bactrocera oleae

dc.contributor.author	Cosmidis, N	en
dc.contributor.author	Loukas, M	en
dc.contributor.author	Peppa, V	en
dc.contributor.author	Goulielmos, G	en
dc.contributor.author	Zouros, E	en
dc.date.accessioned	2014-06-06T06:45:01Z
dc.date.available	2014-06-06T06:45:01Z
dc.date.issued	2002	en
dc.identifier.issn	0018067X	en
dc.identifier.uri	http://dx.doi.org/10.1038/sj.hdy.6800163	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2210
dc.subject	Acetone	en
dc.subject	Alcohol dehydrogenase	en
dc.subject	Bactrocera oleae	en
dc.subject	Multiple forms	en
dc.subject.other	acetone	en
dc.subject.other	Acetone	en
dc.subject.other	Alcohol Dehydrogenase	en
dc.subject.other	Animals	en
dc.subject.other	Gene Frequency	en
dc.subject.other	Isoenzymes	en
dc.subject.other	Survival Analysis	en
dc.subject.other	Tephritidae	en
dc.subject.other	Bactrocera oleae	en
dc.subject.other	Drosophila melanogaster	en
dc.title	Effect of acetone feeding on alcohol dehydrogenase activity in the olive fruit fly, Bactrocera oleae	en
heal.type	journalArticle	en
heal.identifier.primary	10.1038/sj.hdy.6800163	en
heal.publicationDate	2002	en
heal.abstract	The purpose of this study is to demonstrate a clear connection between the presence of acetone in larval diet and alcohol dehydrogenase (ADH) activity in laboratory raised populations of Bactrocera oleae. ADH activity of B. oleae is depressed in acetone-impregnated diets. At the same time the change of activity is accompanied by a change in the relative proportions of the multiple forms of ADH. The bulk of activity in the most cathodally migrating form is lost, and all the activity becomes localized in the less cathodally migrating forms of the enzyme. Moreover, ADH activity, expressed in vivo, appears to drop after exposure to acetone, as shown by the fact that larvae become less sensitive to pentenol poisoning. Our results show clear selective differences imposed by acetone on three homozygous genotypes involving the ADH alleles F, S and I in B. oleae. The directions of these differences were found to vary with the fitness component under test. Acetone treatment seems to affect developmental time and larva's viability as well as allele frequencies of ADH under artificial rearing. The effect of acetone on the maintenance of ADH polymorphism in artificially reared populations of B. oleae is further discussed.	en
heal.journalName	Heredity	en
dc.identifier.issue	6	en
dc.identifier.volume	89	en
dc.identifier.doi	10.1038/sj.hdy.6800163	en
dc.identifier.spage	453	en
dc.identifier.epage	459	en