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Early pregnancy diagnosis in swine by direct radioimmunoassay for progesterone in blood spotted on filter paper

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dc.contributor.author Chadio, S en
dc.contributor.author Xylouri, E en
dc.contributor.author Kalogiannis, D en
dc.contributor.author Michalopoulou, E en
dc.contributor.author Evagelatos, S en
dc.contributor.author Menegatos, I en
dc.date.accessioned 2014-06-06T06:45:01Z
dc.date.available 2014-06-06T06:45:01Z
dc.date.issued 2002 en
dc.identifier.issn 03784320 en
dc.identifier.uri http://dx.doi.org/10.1016/S0378-4320(01)00145-2 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2209
dc.subject Blood-spot en
dc.subject Direct RIA en
dc.subject Pig-pregnancy en
dc.subject Pregnancy diagnosis en
dc.subject Progesterone en
dc.subject.other progesterone en
dc.subject.other animal en
dc.subject.other animal disease en
dc.subject.other article en
dc.subject.other artificial insemination en
dc.subject.other blood en
dc.subject.other estrus en
dc.subject.other female en
dc.subject.other male en
dc.subject.other methodology en
dc.subject.other physiology en
dc.subject.other pregnancy en
dc.subject.other pregnancy test en
dc.subject.other radioimmunoassay en
dc.subject.other reproducibility en
dc.subject.other sensitivity and specificity en
dc.subject.other standard en
dc.subject.other swine en
dc.subject.other time en
dc.subject.other Animal en
dc.subject.other Estrus en
dc.subject.other Female en
dc.subject.other Insemination, Artificial en
dc.subject.other Male en
dc.subject.other Pregnancy en
dc.subject.other Pregnancy Tests, Immunologic en
dc.subject.other Progesterone en
dc.subject.other Radioimmunoassay en
dc.subject.other Reference Standards en
dc.subject.other Reproducibility of Results en
dc.subject.other Sensitivity and Specificity en
dc.subject.other Swine en
dc.subject.other Time Factors en
dc.subject.other Animalia en
dc.subject.other Sus scrofa en
dc.title Early pregnancy diagnosis in swine by direct radioimmunoassay for progesterone in blood spotted on filter paper en
heal.type journalArticle en
heal.identifier.primary 10.1016/S0378-4320(01)00145-2 en
heal.publicationDate 2002 en
heal.abstract A direct radioimmunoassay method for the measurement of progesterone in blood dried on filter paper has been developed for the early pregnancy diagnosis in sows, as well as for monitoring progesterone levels during the oestrous cycle. Pregnancy diagnosis was performed with 95 sows on Days 17-22 after artificial insemination (AI). The cut-off value for pregnancy diagnosis of 7.5 ng/ml was calculated (mean ± 2S.D.) from the progesterone concentrations measured on the same days from non-inseminated animals. There were 85 cases considered pregnant on the basis of progesterone concentration, leaving 10 animals non-pregnant. The accuracy for the positive cases was 98.8%. Two of the 10 sows considered as negative subsequently farrowed, giving an accuracy of 80%. The overall accuracy of the method was 96.8%. The blood-spot assay may be a useful tool for early pregnancy diagnosis in swine, with respect to sampling, simplicity, speed and accuracy. © 2002 Elsevier Science B.V. All rights reserved. en
heal.journalName Animal Reproduction Science en
dc.identifier.issue 1-2 en
dc.identifier.volume 69 en
dc.identifier.doi 10.1016/S0378-4320(01)00145-2 en
dc.identifier.spage 65 en
dc.identifier.epage 72 en


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