Dye-ligand affinity adsorbents for enzyme purification

dc.contributor.author	Labrou, NE	en
dc.date.accessioned	2014-06-06T06:45:01Z
dc.date.available	2014-06-06T06:45:01Z
dc.date.issued	2002	en
dc.identifier.issn	10736085	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2208
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0036157623&partnerID=40&md5=097c77090f4980a72a0d14f57f8d1ac7	en
dc.subject	Affinity chromatography	en
dc.subject	Biomimetic dyes	en
dc.subject	Chlorotriazine dyes	en
dc.subject	Protein purification	en
dc.subject.other	adsorbent	en
dc.subject.other	anthraquinone derivative	en
dc.subject.other	cibacron blue f3ga	en
dc.subject.other	dichlorotriazine dye	en
dc.subject.other	dye	en
dc.subject.other	sepharose	en
dc.subject.other	unclassified drug	en
dc.subject.other	affinity chromatography	en
dc.subject.other	article	en
dc.subject.other	binding affinity	en
dc.subject.other	binding kinetics	en
dc.subject.other	biodegradability	en
dc.subject.other	chemical analysis	en
dc.subject.other	enzyme purification	en
dc.subject.other	high performance liquid chromatography	en
dc.subject.other	immobilization	en
dc.subject.other	protein analysis	en
dc.subject.other	protein purification	en
dc.subject.other	synthesis	en
dc.subject.other	Adsorption	en
dc.subject.other	Animals	en
dc.subject.other	Chromatography, Affinity	en
dc.subject.other	Coloring Agents	en
dc.subject.other	Enzymes	en
dc.subject.other	Humans	en
dc.subject.other	Ligands	en
dc.subject.other	Proteins	en
dc.title	Dye-ligand affinity adsorbents for enzyme purification	en
heal.type	journalArticle	en
heal.publicationDate	2002	en
heal.abstract	Affinity chromatography is widely employed in laboratory and large-scale for the purification of biotherapeutics and diagnostics. Some of the most widely used ligands in affinity chromatography have been several reactive chlorotriazine dyes. In particular, immobilized anthraquinone dyes have found a plethora of applications in affinity chromatography because they are inexpensive, are resistant to chemical and biological degradation, are sterilizable and cleanable in situ, and are readily immobilized to generate affinity adsorbents which display high binding capacity for a broad spectrum of proteins. This article provides detailed protocols on the preparation of a dye-ligand affinity adsorbent. Also, detailed protocols for effective application of these media, emphasizing binding and elution conditions are presented.	en
heal.journalName	Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology	en
dc.identifier.issue	1	en
dc.identifier.volume	20	en
dc.identifier.spage	77	en
dc.identifier.epage	84	en