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Bacteriocin production by Enterococcus faecium FAIR-E 198 in view of its application as adjunct starter in Greek Feta cheese making

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dc.contributor.author Sarantinopoulos, P en
dc.contributor.author Leroy, F en
dc.contributor.author Leontopoulou, E en
dc.contributor.author Georgalaki, MD en
dc.contributor.author Kalantzopoulos, G en
dc.contributor.author Tsakalidou, E en
dc.contributor.author Vuyst, LD en
dc.date.accessioned 2014-06-06T06:44:59Z
dc.date.available 2014-06-06T06:44:59Z
dc.date.issued 2002 en
dc.identifier.issn 01681605 en
dc.identifier.uri http://dx.doi.org/10.1016/S0168-1605(01)00633-X en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2195
dc.subject Enterocin production en
dc.subject Enterococcus faecium en
dc.subject Fermentations en
dc.subject Feta cheese en
dc.subject Listeria en
dc.subject.other bacteriocin en
dc.subject.other article en
dc.subject.other cheese en
dc.subject.other controlled study en
dc.subject.other Enterococcus faecium en
dc.subject.other fermentation en
dc.subject.other food processing en
dc.subject.other Listeria en
dc.subject.other milk en
dc.subject.other nonhuman en
dc.subject.other starter culture en
dc.subject.other temperature en
dc.subject.other Animals en
dc.subject.other Bacteriocins en
dc.subject.other Cheese en
dc.subject.other Enterococcus faecium en
dc.subject.other Fermentation en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Kinetics en
dc.subject.other Milk en
dc.subject.other Temperature en
dc.subject.other Enterococcus en
dc.subject.other Enterococcus faecium en
dc.subject.other Listeria en
dc.title Bacteriocin production by Enterococcus faecium FAIR-E 198 in view of its application as adjunct starter in Greek Feta cheese making en
heal.type journalArticle en
heal.identifier.primary 10.1016/S0168-1605(01)00633-X en
heal.publicationDate 2002 en
heal.abstract Bacteriocin production by Enterococcus faecium FAIR-E 198, isolated from Greek Feta cheese, was studied in batch fermentations, under conditions simulating Feta cheese preparation. Maximum enterocin activity and growth rate was obtained in de Man-Rogosa-Sharpe (MRS) broth at 37 °C with controlled pH 6.5. The enterocin was produced throughout the growth phase of the microorganism, showing primary metabolite kinetics with a peak activity during the mid-exponential phase. The use of skimmed milk as substrate revealed low enterocin activity. When fermentations were performed in skimmed milk in the presence of rennet, CaCl2, and a mixed starter culture, no enterocin activity was observed, although the examined strain grew well under the above conditions. Finally, when E. faecium FAIR-E 198 was applied as adjunct starter in Feta cheese making, no enterocin activity was detected throughout ripening. Results obtained underline the frequently underestimated finding that in vitro production by novel bacteriocinogenic starter or co-cultures is no guarantee for in situ efficiency. It was concluded that the complex food environment thoroughly interferes with bacteriocin production levels. Copyright © 2002 Elsevier Science B.V. en
heal.journalName International Journal of Food Microbiology en
dc.identifier.issue 1-2 en
dc.identifier.volume 72 en
dc.identifier.doi 10.1016/S0168-1605(01)00633-X en
dc.identifier.spage 125 en
dc.identifier.epage 136 en


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