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A non-Mendelian inheritance of resistance to strobilurin fungicides in Ustilago maydis

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dc.contributor.author Ziogas, BN en
dc.contributor.author Markoglou, AN en
dc.contributor.author Tzima, A en
dc.date.accessioned 2014-06-06T06:44:58Z
dc.date.available 2014-06-06T06:44:58Z
dc.date.issued 2002 en
dc.identifier.issn 1526498X en
dc.identifier.uri http://dx.doi.org/10.1002/ps.543 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2183
dc.subject Cytochrome bc1 complex en
dc.subject Non-Mendelian heredity en
dc.subject Qo-inhibitors en
dc.subject Strobilurin resistance en
dc.subject Ustilago maydis en
dc.subject.other acrylic acid derivative en
dc.subject.other antimycin A1 en
dc.subject.other azoxystrobin en
dc.subject.other fungicide en
dc.subject.other ICIA 5504 en
dc.subject.other nitrosoguanidine en
dc.subject.other pyrimidine derivative en
dc.subject.other salicylamide derivative en
dc.subject.other salicylhydroxamic acid en
dc.subject.other strobilurin en
dc.subject.other ubiquinol cytochrome c reductase en
dc.subject.other unclassified drug en
dc.subject.other disease resistance en
dc.subject.other fungicide en
dc.subject.other heritability en
dc.subject.other mutation en
dc.subject.other antibiotic resistance en
dc.subject.other conference paper en
dc.subject.other crop pest en
dc.subject.other cross resistance en
dc.subject.other drug effect en
dc.subject.other electron transport en
dc.subject.other fungal cell en
dc.subject.other fungal gene en
dc.subject.other fungal strain en
dc.subject.other fungicidal activity en
dc.subject.other fungus growth en
dc.subject.other fungus isolation en
dc.subject.other genetic resistance en
dc.subject.other genetics en
dc.subject.other growth, development and aging en
dc.subject.other inheritance en
dc.subject.other metabolism en
dc.subject.other mitochondrial respiration en
dc.subject.other mutagenesis en
dc.subject.other mutation en
dc.subject.other mutational analysis en
dc.subject.other oxygen consumption en
dc.subject.other phenotype en
dc.subject.other plant disease en
dc.subject.other progeny en
dc.subject.other respiratory chain en
dc.subject.other statistical model en
dc.subject.other time en
dc.subject.other Ustilago en
dc.subject.other Ustilago maydis en
dc.subject.other Acrylates en
dc.subject.other Drug Resistance, Fungal en
dc.subject.other Electron Transport Complex III en
dc.subject.other Fungicides, Industrial en
dc.subject.other Genes, Fungal en
dc.subject.other Logistic Models en
dc.subject.other Methacrylates en
dc.subject.other Mutation en
dc.subject.other Pyrimidines en
dc.subject.other Salicylamides en
dc.subject.other Time Factors en
dc.subject.other Ustilago en
dc.subject.other Ustilago en
dc.subject.other Ustilago maydis en
dc.subject.other Zea mays en
dc.title A non-Mendelian inheritance of resistance to strobilurin fungicides in Ustilago maydis en
heal.type conferenceItem en
heal.identifier.primary 10.1002/ps.543 en
heal.publicationDate 2002 en
heal.abstract Mutants of Ustilago maydis (DC) Corda with high resistance to azoxystrobin (RF 164 to 4714, based on EC50 values), an inhibitor of mitochondrial electron transport at the cytochrome bc1 complex, were isolated in a mutation frequency of 2.3 × 10-7 after nitrosoguanidine mutagenesis and selection on media containing 1 μgml-1 azoxystrobin in addition to 0.5 mM salicylhydroxamate (SHAM), a specific inhibitor of cyanide-resistant (alternative) respiration. Oxygen uptake in whole cells was strongly inhibited in the wild-type strains by azoxystrobin (1.5 μgml-1) in addition to SHAM (1 nM), but not in the mutant isolates. Genetic analysis with nine such mutant isolates resulted in progeny phenotypes which did not follow Mendelian segregation, but satisfied the criteria of non-Mendelian (cytoplasmic) heredity. In crosses between three mutant isolates with the compatible wild-type strains, the sensitivity was inherited by progeny maternally from the wild-type parent strain (criterion of uniparental inheritance). In crosses between wild-type strains and remaining mutant isolates, a continuous distribution of sensitivity in the progeny was found (criterion of vegetative segregation). The third criterion of cytoplasmic resistance (criterion of intracellular selection) was fulfilled by experiments on the stability of resistance phenotypes. With two exceptions, a reduction of resistance was observed in the mutant strains when they were grown on inhibitor-free medium. Recovery of the high resistance level was observed after they were returned to the selection medium. Cross-resistance studies with other fungicides, which also inhibit electron transport through complex III of respiratory chain, showed that mutations for resistance to azoxystrobin were also responsible for reduced sensitivity to kresoxim-methyl (RF 18 to 1199) and to antimycin-A (RF 20 to 305), which act at the Qo and Qi sites of the cytochrome bc1 complex, respectively. Studies of the fitness of azoxystrobin-resistant isolates showed that these mutations appeared to be pleiotropic, having significant adverse effects on growth in liquid culture and pathogenicity on young corn plants. © 2002 Society of Chemical Industry. en
heal.journalName Pest Management Science en
dc.identifier.issue 9 en
dc.identifier.volume 58 en
dc.identifier.doi 10.1002/ps.543 en
dc.identifier.spage 908 en
dc.identifier.epage 916 en


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