A non-Mendelian inheritance of resistance to strobilurin fungicides in Ustilago maydis

dc.contributor.author	Ziogas, BN	en
dc.contributor.author	Markoglou, AN	en
dc.contributor.author	Tzima, A	en
dc.date.accessioned	2014-06-06T06:44:58Z
dc.date.available	2014-06-06T06:44:58Z
dc.date.issued	2002	en
dc.identifier.issn	1526498X	en
dc.identifier.uri	http://dx.doi.org/10.1002/ps.543	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2183
dc.subject	Cytochrome bc1 complex	en
dc.subject	Non-Mendelian heredity	en
dc.subject	Qo-inhibitors	en
dc.subject	Strobilurin resistance	en
dc.subject	Ustilago maydis	en
dc.subject.other	acrylic acid derivative	en
dc.subject.other	antimycin A1	en
dc.subject.other	azoxystrobin	en
dc.subject.other	fungicide	en
dc.subject.other	ICIA 5504	en
dc.subject.other	nitrosoguanidine	en
dc.subject.other	pyrimidine derivative	en
dc.subject.other	salicylamide derivative	en
dc.subject.other	salicylhydroxamic acid	en
dc.subject.other	strobilurin	en
dc.subject.other	ubiquinol cytochrome c reductase	en
dc.subject.other	unclassified drug	en
dc.subject.other	disease resistance	en
dc.subject.other	fungicide	en
dc.subject.other	heritability	en
dc.subject.other	mutation	en
dc.subject.other	antibiotic resistance	en
dc.subject.other	conference paper	en
dc.subject.other	crop pest	en
dc.subject.other	cross resistance	en
dc.subject.other	drug effect	en
dc.subject.other	electron transport	en
dc.subject.other	fungal cell	en
dc.subject.other	fungal gene	en
dc.subject.other	fungal strain	en
dc.subject.other	fungicidal activity	en
dc.subject.other	fungus growth	en
dc.subject.other	fungus isolation	en
dc.subject.other	genetic resistance	en
dc.subject.other	genetics	en
dc.subject.other	growth, development and aging	en
dc.subject.other	inheritance	en
dc.subject.other	metabolism	en
dc.subject.other	mitochondrial respiration	en
dc.subject.other	mutagenesis	en
dc.subject.other	mutation	en
dc.subject.other	mutational analysis	en
dc.subject.other	oxygen consumption	en
dc.subject.other	phenotype	en
dc.subject.other	plant disease	en
dc.subject.other	progeny	en
dc.subject.other	respiratory chain	en
dc.subject.other	statistical model	en
dc.subject.other	time	en
dc.subject.other	Ustilago	en
dc.subject.other	Ustilago maydis	en
dc.subject.other	Acrylates	en
dc.subject.other	Drug Resistance, Fungal	en
dc.subject.other	Electron Transport Complex III	en
dc.subject.other	Fungicides, Industrial	en
dc.subject.other	Genes, Fungal	en
dc.subject.other	Logistic Models	en
dc.subject.other	Methacrylates	en
dc.subject.other	Mutation	en
dc.subject.other	Pyrimidines	en
dc.subject.other	Salicylamides	en
dc.subject.other	Time Factors	en
dc.subject.other	Ustilago	en
dc.subject.other	Ustilago	en
dc.subject.other	Ustilago maydis	en
dc.subject.other	Zea mays	en
dc.title	A non-Mendelian inheritance of resistance to strobilurin fungicides in Ustilago maydis	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1002/ps.543	en
heal.publicationDate	2002	en
heal.abstract	Mutants of Ustilago maydis (DC) Corda with high resistance to azoxystrobin (RF 164 to 4714, based on EC50 values), an inhibitor of mitochondrial electron transport at the cytochrome bc1 complex, were isolated in a mutation frequency of 2.3 × 10-7 after nitrosoguanidine mutagenesis and selection on media containing 1 μgml-1 azoxystrobin in addition to 0.5 mM salicylhydroxamate (SHAM), a specific inhibitor of cyanide-resistant (alternative) respiration. Oxygen uptake in whole cells was strongly inhibited in the wild-type strains by azoxystrobin (1.5 μgml-1) in addition to SHAM (1 nM), but not in the mutant isolates. Genetic analysis with nine such mutant isolates resulted in progeny phenotypes which did not follow Mendelian segregation, but satisfied the criteria of non-Mendelian (cytoplasmic) heredity. In crosses between three mutant isolates with the compatible wild-type strains, the sensitivity was inherited by progeny maternally from the wild-type parent strain (criterion of uniparental inheritance). In crosses between wild-type strains and remaining mutant isolates, a continuous distribution of sensitivity in the progeny was found (criterion of vegetative segregation). The third criterion of cytoplasmic resistance (criterion of intracellular selection) was fulfilled by experiments on the stability of resistance phenotypes. With two exceptions, a reduction of resistance was observed in the mutant strains when they were grown on inhibitor-free medium. Recovery of the high resistance level was observed after they were returned to the selection medium. Cross-resistance studies with other fungicides, which also inhibit electron transport through complex III of respiratory chain, showed that mutations for resistance to azoxystrobin were also responsible for reduced sensitivity to kresoxim-methyl (RF 18 to 1199) and to antimycin-A (RF 20 to 305), which act at the Qo and Qi sites of the cytochrome bc1 complex, respectively. Studies of the fitness of azoxystrobin-resistant isolates showed that these mutations appeared to be pleiotropic, having significant adverse effects on growth in liquid culture and pathogenicity on young corn plants. © 2002 Society of Chemical Industry.	en
heal.journalName	Pest Management Science	en
dc.identifier.issue	9	en
dc.identifier.volume	58	en
dc.identifier.doi	10.1002/ps.543	en
dc.identifier.spage	908	en
dc.identifier.epage	916	en