dc.contributor.author | Ziogas, BN | en |
dc.contributor.author | Markoglou, AN | en |
dc.contributor.author | Tzima, A | en |
dc.date.accessioned | 2014-06-06T06:44:58Z | |
dc.date.available | 2014-06-06T06:44:58Z | |
dc.date.issued | 2002 | en |
dc.identifier.issn | 1526498X | en |
dc.identifier.uri | http://dx.doi.org/10.1002/ps.543 | en |
dc.identifier.uri | http://62.217.125.90/xmlui/handle/123456789/2183 | |
dc.subject | Cytochrome bc1 complex | en |
dc.subject | Non-Mendelian heredity | en |
dc.subject | Qo-inhibitors | en |
dc.subject | Strobilurin resistance | en |
dc.subject | Ustilago maydis | en |
dc.subject.other | acrylic acid derivative | en |
dc.subject.other | antimycin A1 | en |
dc.subject.other | azoxystrobin | en |
dc.subject.other | fungicide | en |
dc.subject.other | ICIA 5504 | en |
dc.subject.other | nitrosoguanidine | en |
dc.subject.other | pyrimidine derivative | en |
dc.subject.other | salicylamide derivative | en |
dc.subject.other | salicylhydroxamic acid | en |
dc.subject.other | strobilurin | en |
dc.subject.other | ubiquinol cytochrome c reductase | en |
dc.subject.other | unclassified drug | en |
dc.subject.other | disease resistance | en |
dc.subject.other | fungicide | en |
dc.subject.other | heritability | en |
dc.subject.other | mutation | en |
dc.subject.other | antibiotic resistance | en |
dc.subject.other | conference paper | en |
dc.subject.other | crop pest | en |
dc.subject.other | cross resistance | en |
dc.subject.other | drug effect | en |
dc.subject.other | electron transport | en |
dc.subject.other | fungal cell | en |
dc.subject.other | fungal gene | en |
dc.subject.other | fungal strain | en |
dc.subject.other | fungicidal activity | en |
dc.subject.other | fungus growth | en |
dc.subject.other | fungus isolation | en |
dc.subject.other | genetic resistance | en |
dc.subject.other | genetics | en |
dc.subject.other | growth, development and aging | en |
dc.subject.other | inheritance | en |
dc.subject.other | metabolism | en |
dc.subject.other | mitochondrial respiration | en |
dc.subject.other | mutagenesis | en |
dc.subject.other | mutation | en |
dc.subject.other | mutational analysis | en |
dc.subject.other | oxygen consumption | en |
dc.subject.other | phenotype | en |
dc.subject.other | plant disease | en |
dc.subject.other | progeny | en |
dc.subject.other | respiratory chain | en |
dc.subject.other | statistical model | en |
dc.subject.other | time | en |
dc.subject.other | Ustilago | en |
dc.subject.other | Ustilago maydis | en |
dc.subject.other | Acrylates | en |
dc.subject.other | Drug Resistance, Fungal | en |
dc.subject.other | Electron Transport Complex III | en |
dc.subject.other | Fungicides, Industrial | en |
dc.subject.other | Genes, Fungal | en |
dc.subject.other | Logistic Models | en |
dc.subject.other | Methacrylates | en |
dc.subject.other | Mutation | en |
dc.subject.other | Pyrimidines | en |
dc.subject.other | Salicylamides | en |
dc.subject.other | Time Factors | en |
dc.subject.other | Ustilago | en |
dc.subject.other | Ustilago | en |
dc.subject.other | Ustilago maydis | en |
dc.subject.other | Zea mays | en |
dc.title | A non-Mendelian inheritance of resistance to strobilurin fungicides in Ustilago maydis | en |
heal.type | conferenceItem | en |
heal.identifier.primary | 10.1002/ps.543 | en |
heal.publicationDate | 2002 | en |
heal.abstract | Mutants of Ustilago maydis (DC) Corda with high resistance to azoxystrobin (RF 164 to 4714, based on EC50 values), an inhibitor of mitochondrial electron transport at the cytochrome bc1 complex, were isolated in a mutation frequency of 2.3 × 10-7 after nitrosoguanidine mutagenesis and selection on media containing 1 μgml-1 azoxystrobin in addition to 0.5 mM salicylhydroxamate (SHAM), a specific inhibitor of cyanide-resistant (alternative) respiration. Oxygen uptake in whole cells was strongly inhibited in the wild-type strains by azoxystrobin (1.5 μgml-1) in addition to SHAM (1 nM), but not in the mutant isolates. Genetic analysis with nine such mutant isolates resulted in progeny phenotypes which did not follow Mendelian segregation, but satisfied the criteria of non-Mendelian (cytoplasmic) heredity. In crosses between three mutant isolates with the compatible wild-type strains, the sensitivity was inherited by progeny maternally from the wild-type parent strain (criterion of uniparental inheritance). In crosses between wild-type strains and remaining mutant isolates, a continuous distribution of sensitivity in the progeny was found (criterion of vegetative segregation). The third criterion of cytoplasmic resistance (criterion of intracellular selection) was fulfilled by experiments on the stability of resistance phenotypes. With two exceptions, a reduction of resistance was observed in the mutant strains when they were grown on inhibitor-free medium. Recovery of the high resistance level was observed after they were returned to the selection medium. Cross-resistance studies with other fungicides, which also inhibit electron transport through complex III of respiratory chain, showed that mutations for resistance to azoxystrobin were also responsible for reduced sensitivity to kresoxim-methyl (RF 18 to 1199) and to antimycin-A (RF 20 to 305), which act at the Qo and Qi sites of the cytochrome bc1 complex, respectively. Studies of the fitness of azoxystrobin-resistant isolates showed that these mutations appeared to be pleiotropic, having significant adverse effects on growth in liquid culture and pathogenicity on young corn plants. © 2002 Society of Chemical Industry. | en |
heal.journalName | Pest Management Science | en |
dc.identifier.issue | 9 | en |
dc.identifier.volume | 58 | en |
dc.identifier.doi | 10.1002/ps.543 | en |
dc.identifier.spage | 908 | en |
dc.identifier.epage | 916 | en |
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