| dc.contributor.author |
Pappas, A |
en |
| dc.contributor.author |
Prodromidis, M |
en |
| dc.contributor.author |
Karayannis, M |
en |
| dc.date.accessioned |
2014-06-06T06:44:55Z |
|
| dc.date.available |
2014-06-06T06:44:55Z |
|
| dc.date.issued |
2002 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/S0003-2670(02)00195-2 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2147 |
|
| dc.subject |
Alcohol Dehydrogenase |
en |
| dc.subject |
Flow Injection |
en |
| dc.subject |
Flow Rate |
en |
| dc.subject |
Packed Bed |
en |
| dc.subject |
Sample Size |
en |
| dc.subject |
Controlled Pore Glass |
en |
| dc.subject |
Packed Bed Reactor |
en |
| dc.title |
Flow monitoring of NADH consumption in bioassays based on packed-bed reactors bearing NAD +-dependent dehydrogenases |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/S0003-2670(02)00195-2 |
en |
| heal.publicationDate |
2002 |
en |
| heal.abstract |
An enzymatic method for the individual or simultaneous determination of pyruvic acid and acetaldehyde is described. Alcohol dehydrogenase (ADH) was immobilized onto aminopropyl-modified controlled pore glass, which was then used for the construction of packed-bed (PB) reactors. ADH catalyses the reduction of acetaldehyde to ethanol, in the presence of the coenzyme NADH, which is oxidized to NAD+. Photometric measurements in |
en |
| heal.journalName |
Analytica Chimica Acta |
en |
| dc.identifier.doi |
10.1016/S0003-2670(02)00195-2 |
en |