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Solid state fermentation of broiler litter for production of biocontrol agents

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dc.contributor.author Adams, TT en
dc.contributor.author Eiteman, MA en
dc.contributor.author Hanel, BM en
dc.date.accessioned 2014-06-06T06:44:50Z
dc.date.available 2014-06-06T06:44:50Z
dc.date.issued 2002 en
dc.identifier.issn 09608524 en
dc.identifier.uri http://dx.doi.org/10.1016/S0960-8524(01)00153-5 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2095
dc.subject.other Bacteria en
dc.subject.other Bioassay en
dc.subject.other Biocontrol en
dc.subject.other Cells en
dc.subject.other Methanol en
dc.subject.other Soils en
dc.subject.other Sterilization (cleaning) en
dc.subject.other Pathogens en
dc.subject.other Fermentation en
dc.subject.other beetle en
dc.subject.other biocontrol agent en
dc.subject.other fermentation en
dc.subject.other larva en
dc.subject.other manure en
dc.subject.other soil amendment en
dc.subject.other solid waste en
dc.subject.other animal cell en
dc.subject.other animal experiment en
dc.subject.other article en
dc.subject.other Bacillus thuringiensis en
dc.subject.other bioassay en
dc.subject.other biological control agent en
dc.subject.other cell density en
dc.subject.other fermentation en
dc.subject.other moisture en
dc.subject.other nonhuman en
dc.subject.other poultry en
dc.subject.other priority journal en
dc.subject.other Pseudomonas fluorescens en
dc.subject.other soil en
dc.subject.other sporogenesis en
dc.subject.other wheat en
dc.subject.other wood en
dc.subject.other Animals en
dc.subject.other Bacillus thuringiensis en
dc.subject.other Beetles en
dc.subject.other Biological Assay en
dc.subject.other Biological Products en
dc.subject.other Fermentation en
dc.subject.other Manure en
dc.subject.other Poultry en
dc.subject.other Pseudomonas fluorescens en
dc.subject.other Bacillus thuringiensis en
dc.subject.other Bacillus thuringiensis serovar japonensis en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Coleoptera en
dc.subject.other Japonica en
dc.subject.other Popillia japonica en
dc.subject.other Pseudomonas en
dc.subject.other Pseudomonas fluorescens en
dc.title Solid state fermentation of broiler litter for production of biocontrol agents en
heal.type journalArticle en
heal.identifier.primary 10.1016/S0960-8524(01)00153-5 en
heal.publicationDate 2002 en
heal.abstract Several varieties of heat-sterilized broiler litter with 60% (wet basis, wb) moisture content were substrate in solid-state fermentations to produce biocontrol agents. Litter varieties included litter produced by one flock of broilers from medicated and non-medicated controlled rations, and litter produced by two flocks and four flocks on a single application of bedding material from medicated commercial sources. Litter preparations were inoculated with monocultures of Bacillus thuringiensis serovar japonensis strain Buibui, a pathogen of Japanese beetle larvae (Popillia japonica), or Pseudomonas fluorescens 2-79. B. thuringiensis did not grow in unextracted 1-flock litter nor in water extracted litter, but grew in methanol extracted litter to 5×1010 cell forming units (CFU)/g litter (dry weight, dw) and a spore count of 1×1010CFU/glitter (dw). B. thuringiensis also grew in unprocessed 2-flock and 4-flock litter, achieving cell counts of 3×109 and 1×109CFU/glitter (dw), respectively, and spore counts of 1×109CFU/glitter (dw). P. fluorescens grew in medicated 1-flock litter with no extraction to a cell density greater than 4×1011CFU/glitter (dw). Bioassays in soil containing over 0.5% (db) litter fermented with B. thuringiensis resulted in over 90% mortality in 21 days for first instars of Japanese beetle when compared to a control treatment using compost without fermented litter. The investigations demonstrate that bacterial biocontrol agents produced via solid substrate fermentations using broiler poultry litter have potential in biocontrol applications in the soil environment. Copyright © 2002 . en
heal.journalName Bioresource Technology en
dc.identifier.issue 1 en
dc.identifier.volume 82 en
dc.identifier.doi 10.1016/S0960-8524(01)00153-5 en
dc.identifier.spage 33 en
dc.identifier.epage 41 en


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