| dc.contributor.author |
Eiteman, MA |
en |
| dc.date.accessioned |
2014-06-06T06:44:48Z |
|
| dc.date.available |
2014-06-06T06:44:48Z |
|
| dc.date.issued |
2002 |
en |
| dc.identifier.issn |
10567194 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2082 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-0036570643&partnerID=40&md5=cb5bc934a3afd8011ee3de61aa42f29b |
en |
| dc.subject.other |
Butane |
en |
| dc.subject.other |
Enzymes |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Genes |
en |
| dc.subject.other |
Genetic engineering |
en |
| dc.subject.other |
Growth kinetics |
en |
| dc.subject.other |
Organic acids |
en |
| dc.subject.other |
Substrates |
en |
| dc.subject.other |
Sugars |
en |
| dc.subject.other |
Microbial production |
en |
| dc.subject.other |
Microbiology |
en |
| dc.title |
Genetically engineered E. coli produce succinic acid |
en |
| heal.type |
other |
en |
| heal.publicationDate |
2002 |
en |
| heal.abstract |
The genetic modification of Escherischia coli to produce succinic acid was discussed. A plasmid containing the gene encoding the pyruvate carboxylase enzyme from Rhizobium etli, was introduced in to E. coli strains. The derivative strain AFP111, transformed with plasmid, cultured aerobically during the growth phase followed by an anaerobic production phase and under conditions with low pyruvate carboxylase activity delivered the highest yield. |
en |
| heal.journalName |
Industrial Bioprocessing |
en |
| dc.identifier.issue |
5 |
en |
| dc.identifier.volume |
24 |
en |
| dc.identifier.spage |
6 |
en |
| dc.identifier.epage |
7 |
en |