In vitro plant regeneration of Mammillaria elongata normal and cristate forms

dc.contributor.author	Papafotiou, M	en
dc.contributor.author	Balotis, GN	en
dc.contributor.author	Louka, PT	en
dc.contributor.author	Chronopoulos, J	en
dc.date.accessioned	2014-06-06T06:44:41Z
dc.date.available	2014-06-06T06:44:41Z
dc.date.issued	2001	en
dc.identifier.issn	01676857	en
dc.identifier.uri	http://dx.doi.org/10.1023/A:1010601328667	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2007
dc.subject	Cactaceae	en
dc.subject	Crest	en
dc.subject	Explant seasonal response	en
dc.subject	Fasciation	en
dc.subject	Micropropagation	en
dc.subject	Tissue culture	en
dc.subject.other	Acetic acid	en
dc.subject.other	Growth kinetics	en
dc.subject.other	Plant cell culture	en
dc.subject.other	Plant regeneration	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Cactaceae	en
dc.subject.other	Mammillaria elongata	en
dc.title	In vitro plant regeneration of Mammillaria elongata normal and cristate forms	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1010601328667	en
heal.publicationDate	2001	en
heal.abstract	In the normal form of Mammillaria elongata, shoots were regenerated in vitro, through callus, from tubercle explants excised from the upper part of the branch and cultured on Murashige and Skoog medium (MS) with 1.07 μM α-napthaleneacetic acid (NAA) and 22.20 μM 6-benzylaminopurine (BA). A high percentage of tubercles explants of the M. elongata cristate form, excised from the tip of the branch and cultured on MS with 0.54 μM NAA and 0.44 μM BA or 1.07 μM NAA, responded by initially forming an inflated cristate shoot, which gave cristate and normal shoots, without callus intervention, when transferred on basal MS. Callus formed on cristate tubercles explants gave both cristate and normal shoots when transferred onto basal MS. Normal and cristate shoots were rooted in vitro on MS with 9.84 μM or 0.98 μM indole-3-butyric acid, respectively, and established ex vitro. In both normal and cristate form, the differential response appeared to be associated with the site of the explant excision. The formation of shoots was influenced by the season of culture; i.e., explants excised in October had a higher shoot formation rate than those excised February.	en
heal.journalName	Plant Cell, Tissue and Organ Culture	en
dc.identifier.issue	2	en
dc.identifier.volume	65	en
dc.identifier.doi	10.1023/A:1010601328667	en
dc.identifier.spage	163	en
dc.identifier.epage	167	en