| dc.contributor.author |
Markoglou, AN |
en |
| dc.contributor.author |
Ziogas, BN |
en |
| dc.date.accessioned |
2014-06-06T06:44:40Z |
|
| dc.date.available |
2014-06-06T06:44:40Z |
|
| dc.date.issued |
2001 |
en |
| dc.identifier.issn |
09311785 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1046/j.1439-0434.2001.00676.x |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/1999 |
|
| dc.subject |
Ergosterol biosynthesis inhibitors |
en |
| dc.subject |
Fenpropidin |
en |
| dc.subject |
Fungicide resistance |
en |
| dc.subject |
Genetic control of resistance |
en |
| dc.subject |
Piperidines |
en |
| dc.subject |
Ustilago maydis |
en |
| dc.subject.other |
concentration (parameters) |
en |
| dc.subject.other |
cross resistance |
en |
| dc.subject.other |
culture medium |
en |
| dc.subject.other |
fenpropidin |
en |
| dc.subject.other |
fungicide resistance |
en |
| dc.subject.other |
fungus growth |
en |
| dc.subject.other |
gene segregation |
en |
| dc.subject.other |
genetic analysis |
en |
| dc.subject.other |
genetic strain |
en |
| dc.subject.other |
growth inhibition |
en |
| dc.subject.other |
morpholine |
en |
| dc.subject.other |
mutant |
en |
| dc.subject.other |
piperidine |
en |
| dc.subject.other |
sensitivity |
en |
| dc.subject.other |
ultraviolet irradiation |
en |
| dc.subject.other |
wild relative |
en |
| dc.subject.other |
fungicide |
en |
| dc.subject.other |
genetic analysis |
en |
| dc.subject.other |
mutation |
en |
| dc.subject.other |
pesticide resistance |
en |
| dc.subject.other |
Fungi |
en |
| dc.subject.other |
Myxogastria |
en |
| dc.subject.other |
Ustilago maydis |
en |
| dc.subject.other |
Zea mays |
en |
| dc.title |
Genetic control of resistance to the piperidine fungicide fenpropidin in Ustilago maydis |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1046/j.1439-0434.2001.00676.x |
en |
| heal.publicationDate |
2001 |
en |
| heal.abstract |
Mutants of Ustilago maydis (DC.) Corda, resistant to the piperidine fungicide fenpropidin, were isolated in a mutation frequency of 3.2×l0-5, after UV-irradiation and selection on media containing 75 μg/ml fenpropidin. Genetic analysis with 15 such mutant isolates resulted in the identification of two unlinked chromosomal loci, U/fpd-1 and U/fpd-2. The U/fpd mutations are responsible for moderate resistance levels to fenpropidin (Rf: 42-56 or 15 based on effective concentration causing a 50% reduction in the growth rate (EC50) or minimal inhibitory concentration (MIC) values, respectively). Haploid strains carrying both U/fpd mutations do not exhibit higher levels of resistance to fenpropidin, indicating no additivity of gene effect between non-allelic genes. Cross-resistance studies with other Sterol Biosynthesis inhibitors (SBIs) showed that the U/fpd-mutant isolates exhibited a positive cross-resistance to the piperidine piperalin and to the related morpholine fungicides fenpropimorph and tridemorph, but not to the inhibitors of C-14 demethylase and squalene epoxidase. Crosses between mutants carrying the U/fpd-genes with compatible isolates carrying the U/fpm or U/tdm mutations, which have been identified in previous genetic studies for resistance to morpholine fungicides fenpropimorph and tridemorph, yielded, with the exception of U/fpd-2×U/fpm-2 crosses, a large number of recombinants with wild-type sensitivity, indicating that the mutant genes involved were not allelic. Analysis of progeny from crosses between U/fpd-2 and U/fpm-2 mutants yielded no recombinants with wild-type sensitivity, but a 1:1 progeny segregation was observed at the MIC for the U/fpd-2 isolates, indicating that these genes are alleles of the same locus. A study of the fitness of fenpropidin-resistant isolates showed that the U/fpd mutations do not affect the phytopathogenic fitness-determining characteristics such as growth in liquid culture and pathogenicity on young corn plants. |
en |
| heal.journalName |
Journal of Phytopathology |
en |
| dc.identifier.issue |
9 |
en |
| dc.identifier.volume |
149 |
en |
| dc.identifier.doi |
10.1046/j.1439-0434.2001.00676.x |
en |
| dc.identifier.spage |
551 |
en |
| dc.identifier.epage |
559 |
en |