Gene expression during heat-shock in embryogenic carrot cell lines

dc.contributor.author	Milioni, D	en
dc.contributor.author	Franz, G	en
dc.contributor.author	Sung, R	en
dc.contributor.author	Hatzopoulos, P	en
dc.date.accessioned	2014-06-06T06:44:40Z
dc.date.available	2014-06-06T06:44:40Z
dc.date.issued	2001	en
dc.identifier.issn	01676857	en
dc.identifier.uri	http://dx.doi.org/10.1023/A:1010682727353	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1998
dc.subject	Carrot cell lines	en
dc.subject	Expression	en
dc.subject	Heat-shock	en
dc.subject	HSP90 gene	en
dc.subject	LEA	en
dc.subject	Oleosin	en
dc.subject.other	cell line	en
dc.subject.other	complementary DNA	en
dc.subject.other	gene expression regulation	en
dc.subject.other	genetic similarity	en
dc.subject.other	genetic transcription	en
dc.subject.other	haploidy	en
dc.subject.other	heat shock protein 90	en
dc.subject.other	heat shock	en
dc.subject.other	plant cell	en
dc.subject.other	somatic embryogenesis	en
dc.subject.other	Amino acids	en
dc.subject.other	Degradation	en
dc.subject.other	Genes	en
dc.subject.other	Thermal stress	en
dc.subject.other	Cell lines	en
dc.subject.other	Plant cell culture	en
dc.subject.other	Daucus carota	en
dc.subject.other	Embryophyta	en
dc.subject.other	Eukaryota	en
dc.subject.other	Prokaryota	en
dc.title	Gene expression during heat-shock in embryogenic carrot cell lines	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1010682727353	en
heal.publicationDate	2001	en
heal.abstract	We have isolated an hsp90 gene from carrot (Daucus carota). The deduced amino acid sequence from this cDNA revealed its similarity to the organelle-type HSP90 protein. It has high homology to other plant organelle-isoforms and shows similar homology to both cytoplasmic and prokaryotic HSP90s. To study the regulation of gene expression during heat-shock, two embryo-specific DC8 and DC59 genes, a tubulin gene and an hsp90 gene were monitored in two embryogenic heat-stressed carrot cell lines. The expression of DC8, a LEA and DC59, an oleosin gene, decreased in both cell lines. In addition, there was a progressive degradation of the accumulated messages with time. The expression of hsp90 gene was induced in both cell lines but with a different pattern of transcript accumulation. These results indicate that the haploid cell line responds differently from the diploid cell line and there is differential transcriptional activity in both cell lines during heat-stress.	en
heal.journalName	Plant Cell, Tissue and Organ Culture	en
dc.identifier.issue	3	en
dc.identifier.volume	65	en
dc.identifier.doi	10.1023/A:1010682727353	en
dc.identifier.spage	221	en
dc.identifier.epage	228	en