Effects of vitamin E on urokinase-plasminogen activator receptor expression by bovine neutrophils

dc.contributor.author	Politis, I	en
dc.contributor.author	Hidiroglou, N	en
dc.contributor.author	Cheli, F	en
dc.contributor.author	Baldi, A	en
dc.date.accessioned	2014-06-06T06:44:38Z
dc.date.available	2014-06-06T06:44:38Z
dc.date.issued	2001	en
dc.identifier.issn	00029645	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1989
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0035195202&partnerID=40&md5=2c594de4c4894831bdca0f8526bc4e8f	en
dc.subject.other	alpha tocopherol	en
dc.subject.other	messenger RNA	en
dc.subject.other	urokinase receptor	en
dc.subject.other	animal cell	en
dc.subject.other	animal experiment	en
dc.subject.other	article	en
dc.subject.other	binding site	en
dc.subject.other	blood sampling	en
dc.subject.other	cattle	en
dc.subject.other	cell isolation	en
dc.subject.other	cell membrane	en
dc.subject.other	cell stimulation	en
dc.subject.other	controlled study	en
dc.subject.other	cow	en
dc.subject.other	delivery	en
dc.subject.other	drug effect	en
dc.subject.other	enzyme activity	en
dc.subject.other	extravasation	en
dc.subject.other	female	en
dc.subject.other	injection	en
dc.subject.other	mammary gland	en
dc.subject.other	measurement	en
dc.subject.other	membrane binding	en
dc.subject.other	neutrophil	en
dc.subject.other	nonhuman	en
dc.subject.other	protein expression	en
dc.subject.other	puerperium	en
dc.subject.other	vitamin supplementation	en
dc.subject.other	Animals	en
dc.subject.other	Binding Sites	en
dc.subject.other	Blotting, Northern	en
dc.subject.other	Cattle	en
dc.subject.other	Dietary Supplements	en
dc.subject.other	Female	en
dc.subject.other	Neutrophils	en
dc.subject.other	Pregnancy	en
dc.subject.other	Receptors, Cell Surface	en
dc.subject.other	RNA, Messenger	en
dc.subject.other	Vitamin E	en
dc.subject.other	Animalia	en
dc.subject.other	Bos taurus	en
dc.subject.other	Bovinae	en
dc.subject.other	Rickettsia sp. PAR	en
dc.title	Effects of vitamin E on urokinase-plasminogen activator receptor expression by bovine neutrophils	en
heal.type	journalArticle	en
heal.publicationDate	2001	en
heal.abstract	Objective-To determine the effect of vitamin E supplementation on urokinase-plasminogen activator (u-PA) receptor (u-PAR) expression by neutrophils of dairy cows. Animals-16 healthy Holstein dairy cows. Procedure-16 cows were assigned to 1 of 2 experimental groups: control (no vitamin E supplementation) and vitamin E supplementation. Supplementation of vitamin E started 4 weeks prior to and continued up to 4 weeks after parturition and included oral administration of vitamin E at 3,000 U/cow per day; these cows also received 1 injection of vitamin E (5,000 units), 1 week prior to the expected date of parturition. Blood samples were collected, and neutrophils were isolated weekly throughout the experimental period. The following variables were measured: u-PA (mRNA), total cell-associated u-PA activity, membrane-bound u-PA activity, and free unoccupied u-PA binding sites on the cell membrane of neutrophils. Results-Stimulated neutrophils isolated from cows that received vitamin E supplementation had significantly higher u-PA mRNA and total cell-associated and membrane-bound u-PA activity at postpartum week 1, compared with those of stimulated neutrophils isolated from control cows. There were no differences between groups throughout the whole experimental period in u-PA binding sites of neutrophils. Conclusions and Clinical Relevance-The increased total cell-associated and membrane-bound u-PA activity in neutrophils isolated from cows that received vitamin E may facilitate the ability of neutrophils to extravasate and reach the mammary gland at post-partum week 1. Rapid recruitment of neutrophils is critical for proper defense of the gland.	en
heal.journalName	American Journal of Veterinary Research	en
dc.identifier.issue	12	en
dc.identifier.volume	62	en
dc.identifier.spage	1934	en
dc.identifier.epage	1938	en