Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from leaves of chilli pepper

dc.contributor.author	Kintzios, S	en
dc.contributor.author	Drossopoulos, JB	en
dc.contributor.author	Lymperopoulos, Ch	en
dc.date.accessioned	2014-06-06T06:44:37Z
dc.date.available	2014-06-06T06:44:37Z
dc.date.issued	2001	en
dc.identifier.issn	01676857	en
dc.identifier.uri	http://dx.doi.org/10.1023/A:1011610413177	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1985
dc.subject	Callus culture	en
dc.subject	Capsicum annuum	en
dc.subject	Globular somatic embryos	en
dc.subject	Mineral nutrition	en
dc.subject	Solanaceae	en
dc.subject.other	Concentration (process)	en
dc.subject.other	Growth (materials)	en
dc.subject.other	Sugar (sucrose)	en
dc.subject.other	Inorganic micronutrients	en
dc.subject.other	Vitamins	en
dc.title	Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from leaves of chilli pepper	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1011610413177	en
heal.publicationDate	2001	en
heal.abstract	The effect of different vitamins and inorganic micronutrients on callus growth and the induction and proliferation of somatic embryos from young mature, fully expanded leaves of chilli pepper (Capsicum annuum L.) was investigated. Explants were cultured on a solid Murashige and Skoog (MS) medium supplemented with 8% (w/v) sucrose, 12.9 μM 6-benzyladenine, 9 μM 2,4-dichlorophenoxyacetic acid and 0.5 mg l-1 thiamin.HCl in various combinations of eleven different vitamins. Alternatively, explants were cultured onto a solid medium containing MS macro- and micronutrients except for the salts of Mn, Zn, I, Cu and Co which were added at either the standard MS concentration or at a tenfold increased (Cu, Co) or decreased (Mn, Zn, I) concentration. The results indicated that somatic embryogenesis from pepper leaves is favoured by the addition of nicotinic acid to the culture medium and the increase of copper concentration (an average induction of 70.2 globular embryos/mm2 of explant surface, 9.2% higher than control), without reducing embryo maturation and germination.	en
heal.journalName	Plant Cell, Tissue and Organ Culture	en
dc.identifier.issue	1	en
dc.identifier.volume	67	en
dc.identifier.doi	10.1023/A:1011610413177	en
dc.identifier.spage	55	en
dc.identifier.epage	62	en