Citrate Metabolism by Enterococcus faecalis FAIR-E 229

dc.contributor.author	Sarantinopoulos, P	en
dc.contributor.author	Kalantzopoulos, G	en
dc.contributor.author	Tsakalidou, E	en
dc.date.accessioned	2014-06-06T06:44:35Z
dc.date.available	2014-06-06T06:44:35Z
dc.date.issued	2001	en
dc.identifier.issn	00992240	en
dc.identifier.uri	http://dx.doi.org/10.1128/AEM.67.12.5482-5487.2001	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1967
dc.subject.other	citric acid	en
dc.subject.other	glucose	en
dc.subject.other	lactose	en
dc.subject.other	article	en
dc.subject.other	cheese	en
dc.subject.other	chemistry	en
dc.subject.other	culture medium	en
dc.subject.other	Enterococcus faecalis	en
dc.subject.other	growth, development and aging	en
dc.subject.other	kinetics	en
dc.subject.other	metabolism	en
dc.subject.other	microbiology	en
dc.subject.other	Cheese	en
dc.subject.other	Citric Acid	en
dc.subject.other	Culture Media	en
dc.subject.other	Enterococcus faecalis	en
dc.subject.other	Glucose	en
dc.subject.other	Kinetics	en
dc.subject.other	Lactose	en
dc.subject.other	Support, Non-U.S. Gov't	en
dc.title	Citrate Metabolism by Enterococcus faecalis FAIR-E 229	en
heal.type	journalArticle	en
heal.identifier.primary	10.1128/AEM.67.12.5482-5487.2001	en
heal.publicationDate	2001	en
heal.abstract	Citrate metabolism by Enterococcus faecalis FAIR-E 229 was studied in various growth media containing citrate either in the presence of glucose or lactose or as the sole carbon source. In skim milk (130 mM lactose, 8 mM citrate), cometabolism of citrate and lactose was observed from the first stages of the growth phase. Lactose was stoichiometrically converted into lactate, while citrate was converted into acetate, formate, and ethanol. When de Man-Rogosa-Sharpe (MRS) broth containing lactose (28 mM) instead of glucose was used, E. faecalis FAIR-E 229 catabolized only the carbohydrate. Lactate was the major end product, and small amounts of ethanol were also detected. Increasing concentrations of citrate (10, 40, 70, and 100 mM) added to MRS broth enhanced both the maximum growth rate of E. faecalis FAIR-E 229 and glucose catabolism, although citrate itself was not catabolized. Glucose was converted stoichiometrically into lactate, while small amounts of ethanol were produced as well. Finally, when increasing initial concentrations of citrate (10, 40, 70, and 100 mM) were used as the sole carbon sources in MRS broth without glucose, the main end products were acetate and formate. Small amounts of lactate, ethanol, and acetoin were also detected. This work strongly supports the suggestion that enterococcal strains have the metabolic potential to metabolize citrate and therefore to actively contribute to the flavor development of fermented dairy products.	en
heal.journalName	Applied and Environmental Microbiology	en
dc.identifier.issue	12	en
dc.identifier.volume	67	en
dc.identifier.doi	10.1128/AEM.67.12.5482-5487.2001	en
dc.identifier.spage	5482	en
dc.identifier.epage	5487	en