Characterization of two alcohol dehydrogenase (Adh) loci from the olive fruit fly, Bactrocera (Dacus) oleae and implications for Adh duplication in dipteran insects

dc.contributor.author	Goulielmos, GN	en
dc.contributor.author	Cosmidis, N	en
dc.contributor.author	Loukas, M	en
dc.contributor.author	Tsakas, S	en
dc.contributor.author	Zouros, E	en
dc.date.accessioned	2014-06-06T06:44:35Z
dc.date.available	2014-06-06T06:44:35Z
dc.date.issued	2001	en
dc.identifier.issn	00222844	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1966
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0035142711&partnerID=40&md5=92fcd94bd4eb810c22177f9064ad704e	en
dc.subject	Alcohol dehydrogenase gene	en
dc.subject	Gene duplication	en
dc.subject	Tephritidae	en
dc.subject.other	alcohol dehydrogenase	en
dc.subject.other	amino acid sequence	en
dc.subject.other	animal tissue	en
dc.subject.other	article	en
dc.subject.other	controlled study	en
dc.subject.other	fly	en
dc.subject.other	gene cluster	en
dc.subject.other	gene duplication	en
dc.subject.other	gene locus	en
dc.subject.other	nonhuman	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	phylogeny	en
dc.subject.other	sequence homology	en
dc.subject.other	Alcohol Dehydrogenase	en
dc.subject.other	Amino Acid Sequence	en
dc.subject.other	Animals	en
dc.subject.other	Cloning, Molecular	en
dc.subject.other	Diptera	en
dc.subject.other	DNA	en
dc.subject.other	Evolution, Molecular	en
dc.subject.other	Gene Duplication	en
dc.subject.other	Genes, Insect	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Phylogeny	en
dc.subject.other	Sequence Alignment	en
dc.subject.other	Animalia	en
dc.subject.other	Bactrocera oleae	en
dc.subject.other	Capitata	en
dc.subject.other	Ceratitis	en
dc.subject.other	Ceratitis capitata	en
dc.subject.other	Diptera	en
dc.subject.other	Drosophila melanogaster	en
dc.subject.other	Hexapoda	en
dc.subject.other	Insecta	en
dc.subject.other	Tephritidae	en
dc.title	Characterization of two alcohol dehydrogenase (Adh) loci from the olive fruit fly, Bactrocera (Dacus) oleae and implications for Adh duplication in dipteran insects	en
heal.type	journalArticle	en
heal.publicationDate	2001	en
heal.abstract	We report the cloning and structural characterization of two Adh loci of the olive fruit fly, Bactrocera oleae. Each of the two genes, named Adh1 and Adh2, consists of three exons and two introns for a total length of 1981 and 988 nucleotides, respectively. Their deduced amino acid sequences of 257 and 258 residues exhibit a 77% identity and display the characteristics of the insect ADH enzymes, which belong to the shortchain dehydrogenases/reductases family. The Adh genes of B. oleae are compared to the two genes of the Mediterranean fly, Ceratitis capitata, the only other species of the Tephritidae family in which the Adh genes have been studied. On the basis of amino acid divergence the four genes form two clusters each containing one gene from each species, as expected if there was one duplication event before speciation. On the basis of nucleotide sequence the four sequences form two clusters each containing the two sequences from the same species, as expected if there was a separate duplication event in each species. To help decide between the two alternatives, we compared at both the amino acid and DNA level the Adh genes of five Drosophila species that are known to carry two such genes and observed that, with only one exception at the amino acid level, conspecific loci cluster together. We conclude that the information we have at present does not allow a firm choice between the hypothesis of a single duplication event that occurred before the split of Bactrocera and Ceratitis from their common ancestor and the hypothesis of two independent duplication events, one in each of the two genera.	en
heal.journalName	Journal of Molecular Evolution	en
dc.identifier.issue	1	en
dc.identifier.volume	52	en
dc.identifier.spage	29	en
dc.identifier.epage	39	en