| heal.abstract |
The possible application of some less commonly used in vitro growth regulating compounds is outlined. A number of treatments were applied to determine the best way of inducing in vitro shoot proliferation and rooting on a modified Driver - Kuniyuki [HortScience 19 (1984) 507] basal medium of lemon (Citrus limon (L.) Burm, f. cv. Interdonato) seedlings. 6-Benzyladenine (BA) alone (1, 2 and 4 mg 1-1) and in combination with either orange juice (10%, v/v), silver nitrate (3 mg 1-1), gibberellic acid (GA3) (0.1 mg 1-1 at the establishment stage and 0.5 mg 1-1 at all combinations during the proliferation stage) or abscisic acid (ABA) (0.2 mg 1-1 only at the establishment stage) were used to stimulate shoot formation during the establishment and the proliferation stage. The combination of BA with ABA gave a high rate of shoot formation, while GA3 and silver nitrate enhanced shoot elongation. When these shoots were transferred to the rooting stage, the effect of application of two different auxins (indole-3-butyric acid (IBA) and α-napthaleneacetic acid) was examined, as well as different methods of application (auxin added to the basal medium and auxin application by dipping the base of the explant in auxin solution). Dipping the base of the explants in a 50% ethanol solution of IBA at 1000 mg 1-1 for 5 s resulted in 80% rooting with subsequent 90% survival of these explants, during acclimatization under mist. © 2001 Elsevier Science B.V. |
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