| dc.contributor.author |
Labrou, NE |
en |
| dc.contributor.author |
Bhogal, N |
en |
| dc.contributor.author |
Hurrell, CR |
en |
| dc.contributor.author |
Findlay, JBC |
en |
| dc.date.accessioned |
2014-06-06T06:44:26Z |
|
| dc.date.available |
2014-06-06T06:44:26Z |
|
| dc.date.issued |
2001 |
en |
| dc.identifier.issn |
00219258 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/1876 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-0035851203&partnerID=40&md5=7ba0764fcfc639d84f9e95bd0fb7f6ee |
en |
| dc.subject.other |
Alcohols |
en |
| dc.subject.other |
Binding energy |
en |
| dc.subject.other |
Biological membranes |
en |
| dc.subject.other |
Chemical bonds |
en |
| dc.subject.other |
Mutagenesis |
en |
| dc.subject.other |
Proteins |
en |
| dc.subject.other |
Substitution reactions |
en |
| dc.subject.other |
Binding sites |
en |
| dc.subject.other |
Ligands |
en |
| dc.subject.other |
Biochemistry |
en |
| dc.subject.other |
methionine |
en |
| dc.subject.other |
neurokinin 2 receptor |
en |
| dc.subject.other |
neurokinin A |
en |
| dc.subject.other |
neurokinin A derivative |
en |
| dc.subject.other |
rhodopsin |
en |
| dc.subject.other |
saredutant |
en |
| dc.subject.other |
streptavidin |
en |
| dc.subject.other |
thiol group |
en |
| dc.subject.other |
affinity labeling |
en |
| dc.subject.other |
amino acid substitution |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
binding affinity |
en |
| dc.subject.other |
binding site |
en |
| dc.subject.other |
biotinylation |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
cross linking |
en |
| dc.subject.other |
disulfide bond |
en |
| dc.subject.other |
drug receptor binding |
en |
| dc.subject.other |
genetic complementation |
en |
| dc.subject.other |
molecular model |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
protein binding |
en |
| dc.subject.other |
protein interaction |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
cell line |
en |
| dc.subject.other |
chemistry |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
mutation |
en |
| dc.subject.other |
Arachnida |
en |
| dc.subject.other |
Hexapoda |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Cell Line |
en |
| dc.subject.other |
Genetic Complementation Test |
en |
| dc.subject.other |
Methionine |
en |
| dc.subject.other |
Mutation |
en |
| dc.subject.other |
Neurokinin A |
en |
| dc.subject.other |
Protein Binding |
en |
| dc.subject.other |
Receptors, Neurokinin-2 |
en |
| dc.title |
Interaction of Met297 in the Seventh Transmembrane Segment of the Tachykinin NK2 Receptor with Neurokinin A |
en |
| heal.type |
journalArticle |
en |
| heal.publicationDate |
2001 |
en |
| heal.abstract |
We report the use of thiol chemistry to define specific and reversible disulfide interactions of Cys-substituted NK2 receptor mutants with analogues of neurokinin A (NKA) containing single cysteine substitutions. The NKA analogues were N-biotinylated to facilitate the rapid detection of covalent analogue-receptor interactions utilizing streptavidin reactivity. N-biotinyl-[Tyr1,Cys9]NKA, N-biotinyl-[Tyr 1,Cys10]NKA were both found to reversibly disulfide bond to the NK2 receptor mutant Met297 → Cys. This is consistent with the improved affinities of these particular analogues for the Met297 → Cys receptor as compared with those for the wild-type and Met297 → Leu receptors. In our three-dimensional model, Met297 occupies the equivalent position in helix 7 to the retinal binding Lys296 in rhodopsin. Binding of the NK2 receptor antagonist [3H]SR 48968 and of 125I-NKA was used to characterize additional receptor mutants. It seems that the aromatic residues Trp99 (helix 3), His198 (helix 5), Tyr266, His267, and Phe270 play an important role in NKA binding as structural determinants. The existence of overlapping SR 48968 and NKA binding sites is also evident. These data suggest that the peptide binding site of the NK2R is at least in part formed by residues buried deep within the trans-membrane bundle and that this intramembranous binding domain may correspond to the binding sites for substantially smaller endogenous GPCR ligands. |
en |
| heal.journalName |
Journal of Biological Chemistry |
en |
| dc.identifier.issue |
41 |
en |
| dc.identifier.volume |
276 |
en |
| dc.identifier.spage |
37944 |
en |
| dc.identifier.epage |
37949 |
en |