Glucose repression of xylitol production in Candida tropicalis mixed-sugar fermentations

dc.contributor.author	Kastner, JR	en
dc.contributor.author	Eiteman, MA	en
dc.contributor.author	Lee, SA	en
dc.date.accessioned	2014-06-06T06:44:26Z
dc.date.available	2014-06-06T06:44:26Z
dc.date.issued	2001	en
dc.identifier.issn	01415492	en
dc.identifier.uri	http://dx.doi.org/10.1023/A:1012435413933	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1873
dc.subject	Candida tropicalis	en
dc.subject	Mixed-sugars	en
dc.subject	Repression	en
dc.subject	Respiratory quotient	en
dc.subject	Xylitol	en
dc.subject.other	alcohol	en
dc.subject.other	glucose	en
dc.subject.other	oxidoreductase	en
dc.subject.other	xylitol	en
dc.subject.other	aerobic metabolism	en
dc.subject.other	article	en
dc.subject.other	Candida tropicalis	en
dc.subject.other	enzyme induction	en
dc.subject.other	fermentation	en
dc.subject.other	nonhuman	en
dc.subject.other	oxygen supply	en
dc.subject.other	respiratory quotient	en
dc.title	Glucose repression of xylitol production in Candida tropicalis mixed-sugar fermentations	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1012435413933	en
heal.publicationDate	2001	en
heal.abstract	Glucose repressed xylose utilization in Candida tropicalis pre-grown on xylose until glucose reached approximately 0-5 g 1-1. In fermentations consisting of xylose (93 g 1-1) and glucose (47 g 1-1), xylitol was produced with a yield of 0.65 g g-1 and a specific rate of 0.09 g g-1 h-1, and high concentrations of ethanol were also produced (25 g 1-1). If the initial glucose was decreased to 8 g 1-1, the xylitol yield (0.79 g g-1) and specific rate (0.24 g g-1 h-1) increased with little ethanol formation (<.5 g 1-1). To minimize glucose repression, batch fermentations were performed using an aerobic, glucose growth phase followed by xylitol production. Xylitol was produced under O2 limited and anaerobic conditions, but the specific production rate was higher under O2 limited conditions (0.1-0.4 vs. 0.03 g g-1 h-1). On-line analysis of the respiratory quotient defined the time of xylose reductase induction.	en
heal.journalName	Biotechnology Letters	en
dc.identifier.issue	20	en
dc.identifier.volume	23	en
dc.identifier.doi	10.1023/A:1012435413933	en
dc.identifier.spage	1663	en
dc.identifier.epage	1667	en