Infectivity, predilection sites, and freeze tolerance of Trichinella spp. in experimentally infected sheep

dc.contributor.author	Theodoropoulos, G	en
dc.contributor.author	Kapel, CMO	en
dc.contributor.author	Webster, P	en
dc.contributor.author	Saravanos, L	en
dc.contributor.author	Zaki, J	en
dc.contributor.author	Koutsotolis, K	en
dc.date.accessioned	2014-06-06T06:44:22Z
dc.date.available	2014-06-06T06:44:22Z
dc.date.issued	2000	en
dc.identifier.issn	09320113	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1833
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0033826561&partnerID=40&md5=6a461adc7a10fa6cc1eecf128e98348a	en
dc.subject.other	adolescent	en
dc.subject.other	animal model	en
dc.subject.other	animal tissue	en
dc.subject.other	article	en
dc.subject.other	cold tolerance	en
dc.subject.other	controlled study	en
dc.subject.other	diaphragm	en
dc.subject.other	freezing	en
dc.subject.other	larva	en
dc.subject.other	male	en
dc.subject.other	masseter muscle	en
dc.subject.other	muscle	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.subject.other	sheep	en
dc.subject.other	tongue	en
dc.subject.other	Trichinella	en
dc.subject.other	trichinosis	en
dc.subject.other	Animals	en
dc.subject.other	Diaphragm	en
dc.subject.other	Freezing	en
dc.subject.other	Larva	en
dc.subject.other	Masseter Muscle	en
dc.subject.other	Mice	en
dc.subject.other	Sheep	en
dc.subject.other	Tongue	en
dc.subject.other	Trichinella	en
dc.subject.other	Trichinosis	en
dc.title	Infectivity, predilection sites, and freeze tolerance of Trichinella spp. in experimentally infected sheep	en
heal.type	journalArticle	en
heal.publicationDate	2000	en
heal.abstract	A total of 36 sheep in groups of 4 were inoculated with 9 isolates of Trichinella and euthanized after 10 weeks. Thereafter, numbers of muscle larvae were determined in 13 different muscles/muscle groups. Muscle larvae were found in high numbers in all four sheep inoculated with T. spiralis, in lower numbers in two sheep inoculated with T. pseudospiralis (USA isolate), and in very low numbers in one sheep inoculated with T. pseudospiralis (USSR isolate) and one inoculated with T. britovi. In infections of high and moderate larval intensity, predilection sites of T. spiralis were the masseter muscles, the tongue, and the diaphragm and those of T. pseudospiralis were the masseter muscle and the neck. In low-intensity infections, muscle larvae were detected only in the diaphragm or in pooled muscle samples. For evaluation of the freeze tolerance of the different Trichinella species in sheep-muscle tissue, samples taken from the filet were stored at + 5°, -5°, and -18 °C, respectively. After exposure for 1 and 4 weeks the tissue was digested and the released larvae were inoculated into mice for determination of the reproductive capacity index (RCI). Larvae of both T. spiralis and T. pseudospiralis survived freezing at -5°and -18 °C for 4 weeks.	en
heal.journalName	Parasitology Research	en
dc.identifier.issue	5	en
dc.identifier.volume	86	en
dc.identifier.spage	401	en
dc.identifier.epage	405	en