Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose

dc.contributor.author	Kintzios, S	en
dc.contributor.author	Drossopoulos, JB	en
dc.contributor.author	Lymperopoulos, C	en
dc.date.accessioned	2014-06-06T06:44:17Z
dc.date.available	2014-06-06T06:44:17Z
dc.date.issued	2000	en
dc.identifier.issn	01904167	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1785
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0033783256&partnerID=40&md5=0b0d0a1c74ad07a0d373812eaa47006c	en
dc.subject.other	4 chlorophenoxyacetic acid	en
dc.subject.other	6 n benzyladenine	en
dc.subject.other	cobalt	en
dc.subject.other	copper	en
dc.subject.other	culture medium	en
dc.subject.other	germination	en
dc.subject.other	hybrid	en
dc.subject.other	indoleacetic acid	en
dc.subject.other	iodine	en
dc.subject.other	kinetin	en
dc.subject.other	manganese	en
dc.subject.other	nicotinic acid	en
dc.subject.other	plant growth	en
dc.subject.other	somatic embryogenesis	en
dc.subject.other	sucrose	en
dc.subject.other	thiamine	en
dc.subject.other	zinc	en
dc.subject.other	Rosa	en
dc.title	Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose	en
heal.type	journalArticle	en
heal.publicationDate	2000	en
heal.abstract	The effect of different vitamins and inorganic micronutrients on callus growth and on the induction and proliferation of somatic embryos from young mature, fully expanded leaves of rose (Rosa hybrids L.) was investigated. Explants were cultured onto a solid Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose, 53.5 μM p-chlorophenoxyacetic acid, 4.6 μM Kinetin, and 0.5 mg L-1 thiamin. HCI in combination with either of eleven different vitamins. Alternatively, explants were cultured onto a solid medium containing MS macro- and micronutrients except for the salts of manganese (Mn), zinc (Zn), iodine (I), copper (Cu), and cobalt (Co), which were added at either the standard MS concentration or at a tenfold increased (Cu, Co) or decreased (Mn, Zn, I) concentration. Embryo maturation and germination took place on a MS medium supplemented with 5.2 μM 6-benzyladenine and 5.7 μM 3-indole-acetic acid. The results indicated that somatic embryogenesis from rose leaves was favored by the addition of nicotinic acid and cystein to the culture medium and the increase of Cu concentration, without reducing embryo maturation and germination.	en
heal.journalName	Journal of Plant Nutrition	en
dc.identifier.issue	10	en
dc.identifier.volume	23	en
dc.identifier.spage	1407	en
dc.identifier.epage	1420	en