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Kishk - a dried fermented milk cereal mixture. 2. Assessment of a variety of protein analytical techniques for determining adulteration and proteolysis

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dc.contributor.author Tamime, AY en
dc.contributor.author Barclay, MNI en
dc.contributor.author Law, AJR en
dc.contributor.author Leaver, J en
dc.contributor.author Anifantakis, EM en
dc.contributor.author O'Connor, TP en
dc.date.accessioned 2014-06-06T06:44:02Z
dc.date.available 2014-06-06T06:44:02Z
dc.date.issued 1999 en
dc.identifier.issn 0023-7302 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/1643
dc.subject Kishk en
dc.subject PAGE en
dc.subject SDS-PAGE en
dc.subject RP-HPLC en
dc.subject immunoassay en
dc.subject.classification Food Science & Technology en
dc.subject.other IMMUNOSORBENT-ASSAY ELISA en
dc.subject.other CAPRINE DAIRY-PRODUCTS en
dc.subject.other COWS MILK en
dc.subject.other EWES MILK en
dc.subject.other BOVINE-MILK en
dc.subject.other MONOCLONAL-ANTIBODIES en
dc.subject.other SANDWICH ELISA en
dc.subject.other GOATS MILK en
dc.subject.other OVINE MILK en
dc.subject.other CHEESE en
dc.title Kishk - a dried fermented milk cereal mixture. 2. Assessment of a variety of protein analytical techniques for determining adulteration and proteolysis en
heal.type journalArticle en
heal.language English en
heal.publicationDate 1999 en
heal.abstract A variety of protein assays were applied to the analysis of 25 commercial samples of Lebanese Kishk to detect the adulteration of goat's milk with cow's milk. Polyacrylamide gel electrophoretic (PAGE) methods could detect the degree of adulteration similar to 25 %. Using sodium dodecyl sulphate (SDS-PAGE), reverse phase high performance liquid chromatography (RP-HPLC) and anion-exchange fast protein liquid chromatography (FPLC) failed to detect the degree of adulteration in Kishk due to a very extensive proteolysis that made the distinction of the original caseins very difficult. Moreover, the immunoassay technique (Cortecs method) had limited application for powder products and could not be used effectively to calculate the degree of adulteration of goat's milk Kishk with cow's milk. (C) Inra/Elsevier, Paris. en
heal.publisher EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER en
heal.journalName LAIT en
dc.identifier.issue 3 en
dc.identifier.volume 79 en
dc.identifier.isi ISI:000080855300005 en
dc.identifier.spage 331 en
dc.identifier.epage 339 en


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