| dc.contributor.author |
Ehaliotis, C |
en |
| dc.contributor.author |
Papadopoulou, K |
en |
| dc.contributor.author |
Kotsou, M |
en |
| dc.contributor.author |
Mari, I |
en |
| dc.contributor.author |
Balis, C |
en |
| dc.date.accessioned |
2014-06-06T06:44:01Z |
|
| dc.date.available |
2014-06-06T06:44:01Z |
|
| dc.date.issued |
1999 |
en |
| dc.identifier.issn |
0168-6496 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/1633 |
|
| dc.subject |
olive-mill wastewater |
en |
| dc.subject |
most probable number-polymerase chain reaction |
en |
| dc.subject |
microbial population dynamics |
en |
| dc.subject |
Azotobacter vinelandii |
en |
| dc.subject |
bioremediation |
en |
| dc.subject.classification |
Microbiology |
en |
| dc.subject.other |
POLYMERASE CHAIN-REACTION |
en |
| dc.subject.other |
ANTIBACTERIAL ACTIVITY |
en |
| dc.subject.other |
NITROGENASE ACTIVITY |
en |
| dc.subject.other |
DNA EXTRACTION |
en |
| dc.subject.other |
FIX NITROGEN |
en |
| dc.subject.other |
SOIL |
en |
| dc.subject.other |
GROWTH |
en |
| dc.subject.other |
WATERS |
en |
| dc.subject.other |
ALPECHIN |
en |
| dc.subject.other |
SEQUENCE |
en |
| dc.title |
Adaptation and population dynamics of Azotobacter vinelandii during aerobic biological treatment of olive-mill wastewater |
en |
| heal.type |
journalArticle |
en |
| heal.language |
English |
en |
| heal.publicationDate |
1999 |
en |
| heal.abstract |
Olive-mill wastewater (OMW) has a high organic and polyphenol content and is resistant to biodegradation. Its disposal leads to a major environmental pollution problem in the Mediterranean basin. The detoxification of OMW following inoculation with Azotobacter vinelandii (strain A) was performed for two successive 5-day-period cycles in an aerobic, biowheel-type reactor, under non-sterile conditions. The phytotoxicity of the processed product was reduced by over 90% at the end of both cycles. To exclusively monitor the A. vinelnandii population in the reactor a most probable number-PCR approach was employed and applied daily to serial dilutions of total DNA extracted from reactor samples. PCR sensitivity was independent of the presence of OMW or non-target DNA. The A. vinelandii population dynamics were successfully monitored, showing an initial adaptation period, followed by a sharp population maximum on the fourth day of both cycles (1.6 x 10(8) and 9.6 x 10(7) cells ml(-1) respectively), after a major phytotoxicity decline. N-2 fixation rates were estimated using the acetylene reduction assay and reached a peak during the first 1-2 days of each cycle (36 and 29 nmol C2H2 ml(-1) h(-1) respectively). The data are consistent with an initial physiological adaptation phase, where the presence of phenolic compounds limits A. vinelandii growth but stimulates N2 fixation, followed by a rapid growth phase as phytotoxicity declines. (C) 1999 Published by Elsevier Science B.V. All rights reserved. |
en |
| heal.publisher |
ELSEVIER SCIENCE BV |
en |
| heal.journalName |
FEMS MICROBIOLOGY ECOLOGY |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
30 |
en |
| dc.identifier.isi |
ISI:000083932500003 |
en |
| dc.identifier.spage |
301 |
en |
| dc.identifier.epage |
311 |
en |