Somatic embryogenesis from mature leaves of rose (Rosa sp.)

dc.contributor.author	Kintzios, S	en
dc.contributor.author	Manos, C	en
dc.contributor.author	Makri, O	en
dc.date.accessioned	2014-06-06T06:44:01Z
dc.date.available	2014-06-06T06:44:01Z
dc.date.issued	1999	en
dc.identifier.issn	07217714	en
dc.identifier.uri	http://dx.doi.org/10.1007/s002990050605	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1628
dc.subject	Embryo	en
dc.subject	Genotypic differences	en
dc.subject	Proembryo	en
dc.subject	Rosa	en
dc.subject	Somatic embryogenesis	en
dc.subject.other	1 naphthylacetic acid	en
dc.subject.other	2,4 dichlorophenoxyacetic acid	en
dc.subject.other	4 chlorophenoxyacetic acid	en
dc.subject.other	6 n benzyladenine	en
dc.subject.other	cultivar	en
dc.subject.other	gibberellic acid	en
dc.subject.other	indoleacetic acid	en
dc.subject.other	kinetin	en
dc.subject.other	plant growth	en
dc.subject.other	somatic embryogenesis	en
dc.subject.other	Rosa	en
dc.title	Somatic embryogenesis from mature leaves of rose (Rosa sp.)	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s002990050605	en
heal.publicationDate	1999	en
heal.abstract	Several plant growth regulators (0.3-53.3 μM 6-benzyladenine, 2,4-dichlorophenoxyacetic acid, gibberellic acid, 3-indoleacetic acid, p-chlorophenoxyacetic acid, kinetin and α-naphthylacetic acid), alone or in combination, and culture conditions were tested for their capacity to induce somatic embryogenesis from mature leaf and stem explants of rose (Rosa sp.) of four commercial rose cultivars (Baccara, Mercedes, Ronto and Soraya). Somatic embryos were only induced from mature leaf explants derived from Soraya on Murashige and Skoog (MS) medium supplemented with 53.5 μM p-chlorophenoxyacetic acid and 4.6 μM kinetin, although satisfactory callus induction rates were obtained from all cultivars. After subculturing on the same medium, embryos at various developmental stages (globular, heart and torpedo shaped) were transferred for maturation onto a MS medium supplemented with 5.2 μM 6-benzyladenine and 5.7 μm 3-indoleacetic acid. Germination of mature embryos took place after subculturing them onto medium of the same composition. Plantlets regenerated from embryos and bearing three to four leaves were transferred to a greenhouse.	en
heal.journalName	Plant Cell Reports	en
dc.identifier.issue	6	en
dc.identifier.volume	18	en
dc.identifier.doi	10.1007/s002990050605	en
dc.identifier.spage	467	en
dc.identifier.epage	472	en