Somatic embryogenesis and in vitro rosmarinic acid accumulation in Salvia officinalis and S. fruticosa leaf callus cultures

dc.contributor.author	Kintzios, S	en
dc.contributor.author	Nikolaou, A	en
dc.contributor.author	Skoula, M	en
dc.date.accessioned	2014-06-06T06:44:01Z
dc.date.available	2014-06-06T06:44:01Z
dc.date.issued	1999	en
dc.identifier.issn	07217714	en
dc.identifier.uri	http://dx.doi.org/10.1007/s002990050604	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1627
dc.subject	Rosmarinic acid	en
dc.subject	Salvia fruticosa	en
dc.subject	Salvia officinalis	en
dc.subject	Secondary metabolites	en
dc.subject	Somatic embryogenesis	en
dc.subject.other	callus culture	en
dc.subject.other	in vitro study	en
dc.subject.other	rosmarinic acid	en
dc.subject.other	somatic embryogenesis	en
dc.subject.other	Salvia fruticosa	en
dc.subject.other	Salvia officinalis	en
dc.title	Somatic embryogenesis and in vitro rosmarinic acid accumulation in Salvia officinalis and S. fruticosa leaf callus cultures	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s002990050604	en
heal.publicationDate	1999	en
heal.abstract	The effect of explant age, plant growth regulators and culture conditions on somatic embryogenesis and rosmarinic acid production from leaf explants of Salvia officinalis and S. fruticosa plants collected in Greece was investigated. Embryogenic callus with numerous spherical somatic embryos could be induced on explants derived from both species and cultured for 3 weeks on a Murashige and Skoog (MS) medium supplemented with 1.8-18 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (Kin) or 10.5-21 μM 1-naphthalenacetic acid and 6-benzyladenine. Only explants from young plants (with six to eight leaves) responded to the culture treatments and, in general, low light intensities (50 μmol m-2 s-1) favoured callus formation and induction of somatic embryos. Somatic embryos were further developed on the same medium. Heart-and torpedo-shaped embryos (1-2 mm long) were subcultured on a growth-regulator-free MS medium for maturation. Maximum rosmarinic acid accumulation in S. officinalis and S. fruticosa callus cultured on 4.5 μM 2,4-D and 4.5 μM Kin was 25.9 and 29.0 g/l, respectively.	en
heal.journalName	Plant Cell Reports	en
dc.identifier.issue	6	en
dc.identifier.volume	18	en
dc.identifier.doi	10.1007/s002990050604	en
dc.identifier.spage	462	en
dc.identifier.epage	466	en