Induction of somatic embryogenesis and in vitro flowering from inflorescences of chamomile (Chamomilla recutita L.)

dc.contributor.author	Kintzios, S	en
dc.contributor.author	Michaelakis, A	en
dc.date.accessioned	2014-06-06T06:43:57Z
dc.date.available	2014-06-06T06:43:57Z
dc.date.issued	1999	en
dc.identifier.issn	07217714	en
dc.identifier.uri	http://dx.doi.org/10.1007/s002990050643	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1581
dc.subject	Chamomilla recutita	en
dc.subject	In vitro flowering	en
dc.subject	Inflorescence	en
dc.subject	Plant regeneration	en
dc.subject	Somatic embryogenesis	en
dc.subject.other	1 naphthylacetic acid	en
dc.subject.other	2,4 dichlorophenoxyacetic acid	en
dc.subject.other	6 n benzyladenine	en
dc.subject.other	breeding methods	en
dc.subject.other	callus culture	en
dc.subject.other	flowering	en
dc.subject.other	growth regulation	en
dc.subject.other	kinetin	en
dc.subject.other	micropropagation	en
dc.subject.other	phytohormone	en
dc.subject.other	plant growth	en
dc.subject.other	somatic embryogenesis	en
dc.subject.other	Chamomilla recutita	en
dc.title	Induction of somatic embryogenesis and in vitro flowering from inflorescences of chamomile (Chamomilla recutita L.)	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s002990050643	en
heal.publicationDate	1999	en
heal.abstract	A protocol has been developed for the induction of somatic embryogenesis from flower explants of chamomile (Chamomilla recutita L.). The effects of several plant growth regulators [α-naphthylacetic acid (NAA), 2,4-dichlorophenoxyacetic acid, 6-benzyladenine (BA) and kinetin (Kin), alone or in combination] and the flower type (disk or ray flower) were investigated. Both types of flowers responded to the callus and shoot induction treatments, but formation of globular somatic embryos took place only on disk-flower-derived explants after 2-4 weeks of culture on a Murashige and Skoog (MS) medium supplemented either with 8.87 μM BA and 1.07 μM NAA or with 26.8 μM NAA and 11.5 μM Kin. However, fully developed, cotyledonary-stage somatic embryos could be induced only on the NAA/Kin medium, 10 weeks after culture initiation. Germination of the embryos and plant regeneration took place after subculture for 4-5 weeks onto medium of the same composition. Plantlets regenerated from embryos flowered in vitro on a MS medium supplemented with 8.87 μM BA and 1.07 μM NAA. The significance of the results with respect to chamomile micropropagation and the utilization of wild populations in breeding programs is discussed.	en
heal.journalName	Plant Cell Reports	en
dc.identifier.issue	7-8	en
dc.identifier.volume	18	en
dc.identifier.doi	10.1007/s002990050643	en
dc.identifier.spage	684	en
dc.identifier.epage	690	en