Developmental regulation and spatial expression of a plastidial fatty acid desaturase from Olea europaea

dc.contributor.author	Poghosyan, ZP	en
dc.contributor.author	Haralampidis, K	en
dc.contributor.author	Martsinkovskaya, AI	en
dc.contributor.author	Murphy, DJ	en
dc.contributor.author	Hatzopoulos, P	en
dc.date.accessioned	2014-06-06T06:43:56Z
dc.date.available	2014-06-06T06:43:56Z
dc.date.issued	1999	en
dc.identifier.issn	09819428	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1564
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0033043765&partnerID=40&md5=4289d0acce96def7dc04d72405cb8056	en
dc.subject	ω-3 desaturase	en
dc.subject	fad7	en
dc.subject	In situ hybridisation	en
dc.subject	Jasmonic acid	en
dc.subject	Membrane lipids	en
dc.subject	Olea europaea	en
dc.subject.other	amino acid sequence	en
dc.subject.other	chloroplast	en
dc.subject.other	complementary DNA	en
dc.subject.other	in situ hybridization	en
dc.subject.other	linolenic acid	en
dc.subject.other	methyl jasmonate	en
dc.subject.other	northern blotting	en
dc.subject.other	omega 3 fatty acid desaturase	en
dc.subject.other	oxylipin	en
dc.subject.other	plant development	en
dc.subject.other	protein expression	en
dc.subject.other	trienoic fatty acid	en
dc.title	Developmental regulation and spatial expression of a plastidial fatty acid desaturase from Olea europaea	en
heal.type	journalArticle	en
heal.publicationDate	1999	en
heal.abstract	Trienoic fatty acids fire major components of chloroplast membranes and are also precursors of the oxylipins, such as methyl jasmonate, that play important roles in signal transduction pathways relating to plant development and responses to stress. A cDNA encoding a plastidial ω-3 fatty acid desaturase responsible for trienoic formation has been isolated from a library made from ripening fruits of Olea europaea L. The predicted protein contains 436 amino acid residues including a consensus chloroplast specific transit peptide. Alignment with other desaturase sequences showed strong homology with the plastidial ω-3 desaturases fad7 and fad8. Since fad8 is only expressed at low temperatures and the olive fruit were developing at > 20 °C, it is concluded that the isolated cDNA is most likely to be derived from fad7. Northern hybridisation showed a transient expression of the putative fad7 gene at early stages of drupe (5-7 WAF) and mesocarp (16-19 WAF) development. In situ hybridisation showed particularly prominent expression in the palisade and vascular tissue of young leaves, the embryo sac and transmitting tissue of the carpel, and the tapetum, pollen grains and vascular tissue of anthers. The distinctive spatial, temporal and environmental regulation of the putative fad7 gene is consistent with major roles, not only in thylakoid membrane formation, but also in the provision of α-linolenate-derived signalling molecules that are particularly important in plant tissues involved in transportation and reproduction.	en
heal.journalName	Plant Physiology and Biochemistry	en
dc.identifier.issue	2	en
dc.identifier.volume	37	en
dc.identifier.spage	109	en
dc.identifier.epage	119	en