Oxygen starvation induces cell death in Candida shehatae fermentations of D-xylose, but not D-glucose

dc.contributor.author	Kastner, JR	en
dc.contributor.author	Jones, WJ	en
dc.contributor.author	Roberts, RS	en
dc.date.accessioned	2014-06-06T06:43:48Z
dc.date.available	2014-06-06T06:43:48Z
dc.date.issued	1999	en
dc.identifier.issn	01757598	en
dc.identifier.uri	http://dx.doi.org/10.1007/s002530051462	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1470
dc.subject.other	xylose	en
dc.subject.other	amino acid sequence	en
dc.subject.other	anaerobic growth	en
dc.subject.other	anoxia	en
dc.subject.other	article	en
dc.subject.other	bacterial count	en
dc.subject.other	bacterial growth	en
dc.subject.other	candida	en
dc.subject.other	cell death	en
dc.subject.other	cell viability	en
dc.subject.other	cell volume	en
dc.subject.other	fermentation	en
dc.subject.other	oxygen consumption	en
dc.subject.other	saccharomyces cerevisiae	en
dc.subject.other	starvation	en
dc.subject.other	Aerobiosis	en
dc.subject.other	Anaerobiosis	en
dc.subject.other	Candida	en
dc.subject.other	Colony Count, Microbial	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Glucose	en
dc.subject.other	Oxygen	en
dc.subject.other	Xylose	en
dc.title	Oxygen starvation induces cell death in Candida shehatae fermentations of D-xylose, but not D-glucose	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s002530051462	en
heal.publicationDate	1999	en
heal.abstract	Candida shehatae cells, cultivated on D-glucose and D-xylose, were subjected to a shift from fully aerobic to anaerobic fermentative conditions. After anaerobic conditions were imposed, growth was limited to approximately one doubling or less as C. shehatae rapidly entered a stationary phase of growth. Following the shift to anoxia, cell viability rapidly declined and the total cell volume declined in the D-xylose fermentations. Moreover, the cell volume distribution shifted to smaller volumes. Cell viability, measured by plate counts, declined nine times faster for D-xylose fermentations than for D-glucose fermentations. Anaerobic growth did not occur on either D- glucose or D-xylose. Selected vitamins and amino acids did not stimulate anaerobic growth in C. shehatae, but did enhance anaerobic growth on D- glucose in S. cerevisiae. The decline in cell viability and lack of anaerobic growth by C. shehatae were attributed to oxygen deficiency and not to ethanol inhibition. The results shed light on why C. shehatae anaerobic fermentations are not currently practical and suggest that research directed towards a biochemical understanding of why C. shehatae can not grow anaerobically will yield significant improvements in ethanol fermentations from D-xylose.	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.issue	6	en
dc.identifier.volume	51	en
dc.identifier.doi	10.1007/s002530051462	en
dc.identifier.spage	780	en
dc.identifier.epage	785	en