| dc.contributor.author |
Tsakalidou, E |
en |
| dc.contributor.author |
Anastasiou, R |
en |
| dc.contributor.author |
Papadimitriou, K |
en |
| dc.contributor.author |
Manolopoulou, E |
en |
| dc.contributor.author |
Kalantzopoulos, G |
en |
| dc.date.accessioned |
2014-06-06T06:43:46Z |
|
| dc.date.available |
2014-06-06T06:43:46Z |
|
| dc.date.issued |
1998 |
en |
| dc.identifier.issn |
0168-1656 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/1449 |
|
| dc.subject |
Streptococcus thermophilus |
en |
| dc.subject |
X-prolyl-dipeptidyl aminopeptidase |
en |
| dc.subject |
purification |
en |
| dc.subject.classification |
Biotechnology & Applied Microbiology |
en |
| dc.subject.other |
LACTIS SUBSP CREMORIS |
en |
| dc.subject.other |
LACTOCOCCUS-LACTIS |
en |
| dc.subject.other |
SEQUENCE-ANALYSIS |
en |
| dc.subject.other |
ACID BACTERIA |
en |
| dc.subject.other |
PEPTIDASE |
en |
| dc.subject.other |
CLONING |
en |
| dc.subject.other |
GENE |
en |
| dc.subject.other |
BULGARICUS |
en |
| dc.subject.other |
PROTEINS |
en |
| dc.subject.other |
MUTANTS |
en |
| dc.title |
Purification and characterisation of an intracellular X-prolyl-dipeptidyl aminopeptidase from Streptococcus thermophilus ACA-DC 4 |
en |
| heal.type |
journalArticle |
en |
| heal.language |
English |
en |
| heal.publicationDate |
1998 |
en |
| heal.abstract |
An intracellular X-prolyl-dipeptidyl aminopeptidase from Streptococcus thermophilus ACA-DC 4, isolated from traditional Greek yoghurt, was purified by anion exchange and gel filtration chromatography. A single band of molecular weight of about 80 000 appeared in SDS-PAGE; by gel filtration it was shown that the native enzyme was dimeric. The peptidase showed optimum activity on glycyl-prolyl 4-nitroanilide at pH 7.0 and at 50 degrees C, with K-m = 3.1 mM and V-max = 3500 U mg(-1); over 50 degrees C the enzyme activity declined rapidly. It was inactivated by PMSF; sulfhydryl group reagents and metal chelators had little effect on enzyme activity. (C) 1998 Elsevier Science B.V. |
en |
| heal.publisher |
ELSEVIER SCIENCE BV |
en |
| heal.journalName |
JOURNAL OF BIOTECHNOLOGY |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
59 |
en |
| dc.identifier.isi |
ISI:000072375100005 |
en |
| dc.identifier.spage |
203 |
en |
| dc.identifier.epage |
211 |
en |