Genomic organization, sequence interrelationship, and physical localization using in situ hybridization of two tandemly repeated DNA sequences in the genus Olea

dc.contributor.author	Katsiotis, A	en
dc.contributor.author	Hagidimitriou, M	en
dc.contributor.author	Douka, A	en
dc.contributor.author	Hatzopoulos, P	en
dc.date.accessioned	2014-06-06T06:43:38Z
dc.date.available	2014-06-06T06:43:38Z
dc.date.issued	1998	en
dc.identifier.issn	08312796	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1406
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0032144568&partnerID=40&md5=49ffe985d662d883fd683becf9c11a42	en
dc.subject	In situ hybridization	en
dc.subject	Methylation	en
dc.subject	Oleaceae	en
dc.subject	Phylogenetic relationships	en
dc.subject	Repeated sequences	en
dc.subject.other	chromosomal localization	en
dc.subject.other	clone	en
dc.subject.other	DNA methylation	en
dc.subject.other	genome library	en
dc.subject.other	genome organization	en
dc.subject.other	in situ hybridization	en
dc.subject.other	isoschizomer	en
dc.subject.other	repetitive DNA	en
dc.subject.other	sequence analysis	en
dc.subject.other	sequence homology	en
dc.subject.other	Base Sequence	en
dc.subject.other	DNA, Plant	en
dc.subject.other	Genomic Library	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Repetitive Sequences, Nucleic Acid	en
dc.subject.other	Sequence Alignment	en
dc.subject.other	Sequence Homology, Nucleic Acid	en
dc.subject.other	Species Specificity	en
dc.subject.other	Trees	en
dc.subject.other	Elaeagnus angustifolia	en
dc.subject.other	Embryophyta	en
dc.subject.other	Forsythia	en
dc.subject.other	Jasminum	en
dc.subject.other	Ligustrum	en
dc.subject.other	Olea europaea	en
dc.subject.other	Olea europaea subsp. cuspidata	en
dc.subject.other	Olea oleaster	en
dc.subject.other	Oleaceae	en
dc.subject.other	Parasyringa	en
dc.subject.other	Phillyrea	en
dc.title	Genomic organization, sequence interrelationship, and physical localization using in situ hybridization of two tandemly repeated DNA sequences in the genus Olea	en
heal.type	journalArticle	en
heal.publicationDate	1998	en
heal.abstract	Two tandemly repeated DNA sequences, the 81-bp family and pOS218, have been isolated from a Sau3AI Olea europaea ssp. sativa partial genomic library. Sequencing of the 81-bp element showed the monomer to be between 78 and 84 bases long and to contain 51-58% adenine and thymidine residues. Comparison between the monomers revealed heterogeneity of the sequence primary structure. The clone pOS218 is 218 bases long, and sequence comparison between the two elements revealed that an internal region of the pOS218 repeated DNA sequence had 79% homology to the 81 bp repeat sequence. A breakage-reunion mechanism, involving the CAAAA sequence, could be responsible for the derivation of pOS218 from the 81 bp family element. By using double target in situ hybridization, co-localization of the two sequences on Olea chromosomes was observed. The sequences were present at DAPI stained heterochromatic regions, as major or minor sites having a subtelomeric or interstitial location. Methylation studies using two sets of isoschizomers, Sau3AI-MboI and MspI-HpaII, demonstrated that most cytosine residues in the GATC sites and the internal cytosine in the CCGG sites of both elements were methylated in O. europaea ssp. sativa. No major difference in methylation was apparent between DNA extracted from young leaves or from callus of O. europaea ssp. sativa. Both elements are also present in Olea chrysophylla, Olea oleaster, and Olea africana, but are absent from other Oleaceae genera, including Phillyrea, Forsythia, Ligustrum, Parasyringa, and Jasminum.	en
heal.journalName	Genome	en
dc.identifier.issue	4	en
dc.identifier.volume	41	en
dc.identifier.spage	527	en
dc.identifier.epage	534	en