| dc.contributor.author |
Hong, HP |
en |
| dc.contributor.author |
Ross, JHE |
en |
| dc.contributor.author |
Gerster, JL |
en |
| dc.contributor.author |
Rigas, S |
en |
| dc.contributor.author |
Datla, RSS |
en |
| dc.contributor.author |
Hatzopoulos, P |
en |
| dc.contributor.author |
Scoles, G |
en |
| dc.contributor.author |
Keller, W |
en |
| dc.contributor.author |
Murphy, DJ |
en |
| dc.contributor.author |
Robert, LS |
en |
| dc.date.accessioned |
2014-06-06T06:43:27Z |
|
| dc.date.available |
2014-06-06T06:43:27Z |
|
| dc.date.issued |
1997 |
en |
| dc.identifier.issn |
01674412 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1023/A:1005840824926 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/1282 |
|
| dc.subject |
β-glucuronidase |
en |
| dc.subject |
Anther |
en |
| dc.subject |
Brassica |
en |
| dc.subject |
Oleosin |
en |
| dc.subject |
Promoter |
en |
| dc.subject |
Tapetum |
en |
| dc.subject.other |
beta glucuronidase |
en |
| dc.subject.other |
recombinant protein |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
biosynthesis |
en |
| dc.subject.other |
Brassica |
en |
| dc.subject.other |
comparative study |
en |
| dc.subject.other |
gene |
en |
| dc.subject.other |
gene expression regulation |
en |
| dc.subject.other |
genetic variability |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
molecular genetics |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
promoter region |
en |
| dc.subject.other |
reporter gene |
en |
| dc.subject.other |
sequence alignment |
en |
| dc.subject.other |
sequence homology |
en |
| dc.subject.other |
transgenic plant |
en |
| dc.subject.other |
Base Sequence |
en |
| dc.subject.other |
Brassica |
en |
| dc.subject.other |
Gene Expression Regulation, Plant |
en |
| dc.subject.other |
Genes, Plant |
en |
| dc.subject.other |
Genes, Reporter |
en |
| dc.subject.other |
Glucuronidase |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Plants, Genetically Modified |
en |
| dc.subject.other |
Promoter Regions (Genetics) |
en |
| dc.subject.other |
Recombinant Proteins |
en |
| dc.subject.other |
Sequence Alignment |
en |
| dc.subject.other |
Sequence Homology, Nucleic Acid |
en |
| dc.subject.other |
Variation (Genetics) |
en |
| dc.subject.other |
Brassica |
en |
| dc.subject.other |
Brassica napus |
en |
| dc.title |
Promoter sequences from two different Brassica napus tapetal oleosin-like genes direct tapetal expression of β-glucuronidase in transgenic Brassica plants |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1023/A:1005840824926 |
en |
| heal.publicationDate |
1997 |
en |
| heal.abstract |
To investigate the sequences responsible for the regulated expression of tapetal-specific oleosin-like genes, ca. 2 kb of the 5'-upstream regions from two divergent genes, OlnB;4 and OlnB;13, were isolated, sequenced and fused to the reporter gene β-glucuronidase for study in transgenic Brassica napus plants. Although the proteins encoded by these two genes are highly divergent, except for the conserved oleosin-like domain, the first 250 bp of their 5'-upstream regions was 86% identical, including a region of 150 hp upstream from the TATA box. Analysis of 42 independent transformants by histochemical anti fluorometric methods showed that both promoters directed tapetal-specific expression that peaked at the 4 mm flower bud stage. |
en |
| heal.journalName |
Plant Molecular Biology |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
34 |
en |
| dc.identifier.doi |
10.1023/A:1005840824926 |
en |
| dc.identifier.spage |
549 |
en |
| dc.identifier.epage |
555 |
en |