| dc.contributor.author |
Beljebbar, A |
en |
| dc.contributor.author |
Morjani, H |
en |
| dc.contributor.author |
Angiboust, JF |
en |
| dc.contributor.author |
Sockalingum, GD |
en |
| dc.contributor.author |
Polissiou, M |
en |
| dc.contributor.author |
Manfait, M |
en |
| dc.date.accessioned |
2014-06-06T06:43:24Z |
|
| dc.date.available |
2014-06-06T06:43:24Z |
|
| dc.date.issued |
1997 |
en |
| dc.identifier.issn |
03770486 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/1244 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-0001642012&partnerID=40&md5=06e46539ce6a7affe50d90344e6de9f6 |
en |
| dc.title |
Molecular and cellular interaction of the differentiating antitumour agent dimethylcrocetin with nuclear retinoic acid receptor as studied by near-infrared and visible SERS spectroscopy |
en |
| heal.type |
journalArticle |
en |
| heal.publicationDate |
1997 |
en |
| heal.abstract |
Fourier transform (FT) Raman and surface enhanced Raman microspectroscopy (SERS) were used as a vibrational probe for investigating, in vitro and at the cellular level, the molecular interaction of dimethylcrocetin (DMCRT) with the retinoic acid nuclear receptor RAR-γ. FT-SERS results obtained in vitro with silver colloids demonstrated that DMCRT interacts specifically with RAR-γ. Comparison between the spectra of DMCRT in HL60 cancer cells and the in vitro DMCRT-RARγ complex showed practically the same shift in wavenumber of the band at 1210 cm-1 and the same intensity ratios I1541/I1165 and I1541/I1210. The similarity between the signal of DMCRT in K562 cells and in the free form is explained by either an absence of nuclear receptor or an absence of any interaction between the drug and receptor. These results seem to indicate that carotenoids could possibly activate the nuclear receptor in a similar manner to retinoids. © 1997 by John Wiley & Sons, Ltd. |
en |
| heal.journalName |
Journal of Raman Spectroscopy |
en |
| dc.identifier.issue |
2-3 |
en |
| dc.identifier.volume |
28 |
en |
| dc.identifier.spage |
159 |
en |
| dc.identifier.epage |
163 |
en |