| dc.contributor.author |
Kisaalita, WS |
en |
| dc.date.accessioned |
2014-06-06T06:43:15Z |
|
| dc.date.available |
2014-06-06T06:43:15Z |
|
| dc.date.issued |
1997 |
en |
| dc.identifier.issn |
10979336 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/1131 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-0030702930&partnerID=40&md5=9b87d458f94e23de1a0c8003189e1b4e |
en |
| dc.subject.other |
acetylsalicylic acid |
en |
| dc.subject.other |
ascorbic acid |
en |
| dc.subject.other |
edetic acid |
en |
| dc.subject.other |
retinoic acid |
en |
| dc.subject.other |
teratogenic agent |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cell aging |
en |
| dc.subject.other |
cell culture |
en |
| dc.subject.other |
cell death |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
cytotoxicity |
en |
| dc.subject.other |
electrophysiology |
en |
| dc.subject.other |
mouse |
en |
| dc.subject.other |
nerve cell differentiation |
en |
| dc.subject.other |
neuroblastoma cell |
en |
| dc.subject.other |
nonhuman |
en |
| dc.title |
Susceptibility of differentiating neuroblastoma cells to developmental toxicants' cytotoxic effects is culture age-dependent |
en |
| heal.type |
journalArticle |
en |
| heal.publicationDate |
1997 |
en |
| heal.abstract |
The cytotoxic effects of developmental (teratogenic) and nondevelopmental toxicants on neuroblastoma cells at various times during electrophysiological differentiation were evaluated. A N1E-115 cell-line, a clone of the murine neuroblastoma C1300 derived from the neural crest, was exposed to retinoic acid, acetylsalicylic acid (ASA), ascorbic acid, and ethyldiaminetetraacetate (EDTA). These differentiating N1E-115 cells were exposed to the toxicants at EC50 concentrations. Cells exposed to the known developmental toxicants (retinoic acid and ASA) exhibited the greatest vulnerability in terms of cell death during the 8 to 10-day period. A similar gestation age-dependent sensitivity with respect to induction of gross malformations has been reported in rodents. Cells exposed to nondevelopmental toxicants (ascorbic acid and EDTA) did not exhibit any distinct period of greatest vulnerability. These results show that differentiating N1E-115 culture age-dependent vulnerability in not limited to compounds with Vitamin A-like activities (retinoids) and further show time-dependent similarities between the in vivo and in vitro efects. |
en |
| heal.journalName |
In Vitro and Molecular Toxicology: Journal of Basic and Applied Research |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
10 |
en |
| dc.identifier.spage |
359 |
en |
| dc.identifier.epage |
363 |
en |