Kinetic fluorometry of the iron-pyoverdine complex in acetate buffer media

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dc.contributor.author Kisaalita, WS en
dc.contributor.author Slininger, PJ en
dc.contributor.author Bothast, RJ en
dc.date.accessioned 2014-06-06T06:43:15Z
dc.date.available 2014-06-06T06:43:15Z
dc.date.issued 1997 en
dc.identifier.issn 0951208X en
dc.relation.uri http://www.scopus.com/inward/record.url?eid=2-s2.0-0030820366&partnerID=40&md5=dffec1a563a2936d7e7cb6c747b4e2fe en
dc.subject.other Pseudomonas en
dc.subject.other Pseudomonas fluorescens en
dc.title Kinetic fluorometry of the iron-pyoverdine complex in acetate buffer media en
heal.type journalArticle en
heal.publicationDate 1997 en
heal.abstract In the presence of iron and acetate, the rate of increase of Pseudomonas fluorescens pyoverdine fluorescence was inversely proportional to the iron concentration, up to approximately 200 μg/l. An assay based on this observation would however require calibration curves to be generated in a matrix similar to that of the sample. Pyoverdine has an affinity for Fe(II), suggesting that it may be necessary to convert Fe(II) to Fe(III) prior to assay. More detailed work regarding the mechanism underlying the change in fluorescence, assay specificity, accuracy and precision is necessary to establish this method as a new analytical technique. en
heal.journalName Biotechnology Techniques en
dc.identifier.issue 9 en
dc.identifier.volume 11 en
dc.identifier.spage 649 en
dc.identifier.epage 651 en

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