Kinetic fluorometry of the iron-pyoverdine complex in acetate buffer media

dc.contributor.author	Kisaalita, WS	en
dc.contributor.author	Slininger, PJ	en
dc.contributor.author	Bothast, RJ	en
dc.date.accessioned	2014-06-06T06:43:15Z
dc.date.available	2014-06-06T06:43:15Z
dc.date.issued	1997	en
dc.identifier.issn	0951208X	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/1123
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0030820366&partnerID=40&md5=dffec1a563a2936d7e7cb6c747b4e2fe	en
dc.subject.other	Pseudomonas	en
dc.subject.other	Pseudomonas fluorescens	en
dc.title	Kinetic fluorometry of the iron-pyoverdine complex in acetate buffer media	en
heal.type	journalArticle	en
heal.publicationDate	1997	en
heal.abstract	In the presence of iron and acetate, the rate of increase of Pseudomonas fluorescens pyoverdine fluorescence was inversely proportional to the iron concentration, up to approximately 200 μg/l. An assay based on this observation would however require calibration curves to be generated in a matrix similar to that of the sample. Pyoverdine has an affinity for Fe(II), suggesting that it may be necessary to convert Fe(II) to Fe(III) prior to assay. More detailed work regarding the mechanism underlying the change in fluorescence, assay specificity, accuracy and precision is necessary to establish this method as a new analytical technique.	en
heal.journalName	Biotechnology Techniques	en
dc.identifier.issue	9	en
dc.identifier.volume	11	en
dc.identifier.spage	649	en
dc.identifier.epage	651	en