| dc.contributor.author |
Somers, DJ |
en |
| dc.contributor.author |
Zhou, Z |
en |
| dc.contributor.author |
Bebeli, PJ |
en |
| dc.contributor.author |
Gustafson, JP |
en |
| dc.date.accessioned |
2014-06-06T06:43:13Z |
|
| dc.date.available |
2014-06-06T06:43:13Z |
|
| dc.date.issued |
1996 |
en |
| dc.identifier.issn |
00405752 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/1105 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-0030461788&partnerID=40&md5=75d28734bb762c92935352646ab2f42d |
en |
| dc.subject |
DAMD |
en |
| dc.subject |
Genome-specific |
en |
| dc.subject |
Minisatellite |
en |
| dc.subject |
PCR |
en |
| dc.subject |
Triticum |
en |
| dc.subject |
Wheat |
en |
| dc.subject.other |
Triticum aestivum |
en |
| dc.title |
Repetitive, genome-specific probes in wheat (Triticum aestivum L. em Thell) amplified with minisatellite core sequences |
en |
| heal.type |
journalArticle |
en |
| heal.publicationDate |
1996 |
en |
| heal.abstract |
The detection and analysis of DNA polymorphisms in crops is an essential component of marker-assisted selection and cultivar identification in plant breeding. We have explored the direct amplification of minisatellite DNA by PCR (DAMD-PCR) as a means for generating DNA probes that are useful for detecting DNA polymorphisms and DNA fingerprinting in wheat. This technique was facilitated by high-stringency PCR with known plant and animal minisatellite core sequences as primers on wheat genomic DNA. The products of DAMD-PCR from Triticum aestivum, T. durum, T. monococcum, T. speltoides and T. tauschii showed a high degree of polymorphism and the various genomes could be identified. Cloning of the DAMD-PCR products and subsequent Southern hybridization frequently revealed polymorphic probes showing a good degree of genome specificity. In addition, polymorphic, single locus, and moderately dispersed PCR products were cloned that may have a potential for DNA fingerprinting. Our experiments were limited primarily to diploid wheats and the results indicated that DAMD-PCR may isolate genome-specific probes from wild diploid wheat species that could be used to monitor genome introgression into hexaploid wheat. |
en |
| heal.journalName |
Theoretical and Applied Genetics |
en |
| dc.identifier.issue |
5-6 |
en |
| dc.identifier.volume |
93 |
en |
| dc.identifier.spage |
982 |
en |
| dc.identifier.epage |
989 |
en |