| heal.abstract |
Callus cultures were initiated from mature excised embryos received from seeds of the Triticum aestivum L. cvs. 'Aeges' and 'Genereso' and the Triticum durum L. cvs. 'Mexicali' and 'Capeiti'. The embryos were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of growth regulators. The four cultivars differed remarkably in their response to embryo culture with various growth regulators, with the T. durum cultivar 'Capeiti' being the least responsive. No callus growth was observed when no growth regulator was applied and maximum callus growth was obtained on a medium supplemented with 2,4-D and kinetin. For both T. aestivum and T. durum cultivars, better plant regeneration rates were obtained by inducing callus formation, shoot induction and root induction on a single medium, rather than submitting explants to four separate culture phases. Addition of 1 mg 1-1 NAA to the medium was beneficial to root and shoot induction in all cases. T. aestivum callus rooted better when NAA was used alone or in combination with 2iP, while application of NAA and BA proved to be the best PGR combination for the T. durum cultivars. The combination of 2,4- D with kinetin, which improved callusing, had a rather adverse effect on root and shoot induction. |
en |